DNCIC1 Antibody (74.1)

Images

 
Western Blot: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of cytoplasmic dynein in HeLa cell lysate.
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without ...read more
Immunohistochemistry-Paraffin: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in human colon tissue using anti-DNCIC1 antibody. Image from verified customer review.
Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in C6 cells compared to an isotype control (blue).
Western Blot: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in human colon cancer cell lysates using anti-DNCIC1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in caco-2 cells using anti-DNCIC1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without ...read more
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in U87-MG Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed ...read more
Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in U87-MG cells compared to an isotype control (blue).
Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in Hela cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Dr, Ma, XpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P, IP
Clone
74.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DNCIC1 Antibody (74.1) Summary

Immunogen
Purified bovine brain cytoplasmic dynein.
Localization
Cytoplasmic
Specificity
Detects two isoforms of the 74kDa polypeptide of cytoplasmic Dynein.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
DYNC1I1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 1 - 2 ug
  • Immunocytochemistry/Immunofluorescence 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB300-726 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for DNCIC1 Antibody (74.1)

  • cytoplasmic dynein 1 intermediate chain 1
  • Cytoplasmic dynein intermediate chain 1
  • DNCI1cytoplasmic, intermediate polypeptide 1
  • DNCIC1DH IC-1
  • Dynein intermediate chain 1, cytosolic
  • dynein, cytoplasmic 1, intermediate chain 1

Background

The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two 530 kDa heavy chains, and intermediate chains of ~74 kDa and 53-59 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for DNCIC1 Antibody (NB300-726) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 3 application: IHC-P, IF, WB.

Reviews using NB300-726:
Filter by Applications
IHC-P
(1)
IF
(1)
WB
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin DNCIC1 NB300-726
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reviewed by:
Anonymous
IHC-P Human 03/25/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman colon tissue
SpeciesHuman
Immunofluorescence DNCIC1 NB300-726
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reviewed by:
Anonymous
IF Human 03/23/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedCaco-2 cell line
SpeciesHuman

Blocking

Blocking DetailsNo blocking

Primary Anitbody

Dilution RatioDilution: 1/400 in 1xPBS, 1 hour at room temperature

Secondary Antibody

Secondary DescriptionCy3 anti-mouse

Details

Detection NotesImmunoflourescence microscopy
Fixation DetailsMethanol-Aceton fixation
Wash Description3 x 5 min. washing with 1xPBS
Western Blot DNCIC1 NB300-726
Enlarge
reviewed by:
Anonymous
WB Human 03/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman colon cancer cell lysates
SpeciesHuman

Blocking

Blocking Details1 hour, at room temperature, in 5% non-fat milk powder solution (prepared with 1xPBS)

Primary Anitbody

Dilution RatioDilution:1/1000, 1 hour incubation at room temperature

Secondary Antibody

Secondary DescriptionHRP

Details

Detection NotesEnhanced chemiluminescence (ECL) detection method, exposure time: 30 sec-1 minute

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNCIC1 Antibody (NB300-726). (Showing 1 - 1 of 1 FAQ).

  1. Is the DNCIC1 antibody with the number NB300-726 is only specific for DNCIC1 protein? Does it detect the DNCIC2 protein as well?
    • For our product NB300-726, we have not yet tested this product for cross reactivity against the DNCIC2 protein. These two proteins share quite a few regions of 100% homology, so I think it is highly likely that this product would cross react with the DNCIC2 protein.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DNCIC1 Antibody (NB300-726)

Discover related pathways, diseases and genes to DNCIC1 Antibody (NB300-726). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for DNCIC1 Antibody (NB300-726)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
03/25/2015
Application: IHC-P
Species: Human

Anonymous
03/23/2015
Application: IF
Species: Human

Anonymous
03/23/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol DYNC1I1
Entrez
Uniprot