DNCIC1 Antibody (74.1)

Western Blot: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of cytoplasmic dynein in HeLa cell lysate.

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in human colon tissue using anti-DNCIC1 antibody. Image from verified customer review.

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in C6 cells compared to an isotype control (blue).

Western Blot: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in human colon cancer cell lysates using anti-DNCIC1 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in caco-2 cells using anti-DNCIC1 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in U87-MG Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in U87-MG cells compared to an isotype control (blue).

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in Hela cells compared to an isotype control (blue).

• Reactivity: Hu, Mu, Rt, Dr, Ma, Xp
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 74.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DNCIC1 Antibody (74.1) Summary

• Description: Novus Biologicals Mouse DNCIC1 Antibody (74.1) (NB300-726) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified bovine brain cytoplasmic dynein.
• Localization: Cytoplasmic
• Specificity: Detects two isoforms of the 74kDa polypeptide of cytoplasmic Dynein.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DYNC1I1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 - 2 ug
  • Immunocytochemistry/ Immunofluorescence 2 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DNCIC1 Antibody (74.1)

• cytoplasmic dynein 1 intermediate chain 1
• Cytoplasmic dynein intermediate chain 1
• DNCI1cytoplasmic, intermediate polypeptide 1
• DNCIC1DH IC-1
• Dynein intermediate chain 1, cytosolic
• dynein, cytoplasmic 1, intermediate chain 1

Background

The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two 530 kDa heavy chains, and intermediate chains of ~74 kDa and 53-59 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for DNCIC1 Antibody (NB300-726) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 03/25/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human colon tissue
• Species: Human

reviewed by: Verified Customer IF Human 03/23/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Caco-2 cell line
• Species: Human

reviewed by: Verified Customer WB Human 03/23/2015

Summary

• Application: Western Blot
• Sample Tested: Human colon cancer cell lysates
• Species: Human

FAQs for DNCIC1 Antibody (NB300-726). (Showing 1 - 1 of 1 FAQ).

1. Is the DNCIC1 antibody with the number NB300-726 is only specific for DNCIC1 protein? Does it detect the DNCIC2 protein as well?
   • For our product NB300-726, we have not yet tested this product for cross reactivity against the DNCIC2 protein. These two proteins share quite a few regions of 100% homology, so I think it is highly likely that this product would cross react with the DNCIC2 protein.

Bioinformatics

• Gene Symbol: DYNC1I1
• Entrez:
  • 1780(Human)
• Uniprot:
  • O14576(Human)