Cathepsin S Antibody [Unconjugated]

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Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Cathepsin S Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331
Accession # P25774
Specificity
Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSS
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.2-1.8 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human Cathepsin S Antibody (Catalog # AF1183)
ELISA Detection: Human Cathepsin S Biotinylated Antibody (Catalog # BAF1183)
Standard: Recombinant Human Cathepsin S (Catalog # 1183-CY)
Publications
Read Publications using
AF1183 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin S Antibody [Unconjugated]

  • Cathepsin S
  • CTSS
  • EC 3.4.22
  • EC 3.4.22.27
  • FLJ50259
  • MGC3886

Background

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1-16), a pro region (residues 17-114), and the mature enzyme (residues 115-331) (3-5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages, and other phagocytic cells.

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin S Antibody (AF1183)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 5 applications: Flow, IHC Paraffin-embedded, IHC-OCT embedded, Luminex Development, WB.


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Flow
(1)
IHC Paraffin-embedded
(3)
IHC-OCT embedded
(1)
Luminex Development
(1)
WB
(7)
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Filter By Species
Human
(9)
Mouse
(3)
Chicken
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF1183 Applications Species
The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity PLoS ONE, 2016;11(8):e0161573. 2016 [PMID: 27561012] (WB,, Chicken) WB Chicken
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors Sci Rep, 2016;6(0):29256. 2016 [PMID: 27387133] (WB,, Human) WB Human
Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer Oncotarget, 2016;7(19):28124-38. 2016 [PMID: 27058412] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases J. Biol. Chem., 2016;291(16):8486-99. 2016 [PMID: 26884336] (WB,, Human) WB Human
Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE, 2015;10(4):e0120348. 2015 [PMID: 25927437] (IHC-OCT embedded,, Mouse) IHC-OCT embedded Mouse
Barry, Zachary, Platt, Manu O Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation. J Biol Chem, 2012;287(33):27723-30. 2012 [PMID: 22730330] (WB,, Human) WB Human
Kwok HF, Buick RJ, Kuehn D Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity. Mol. Cancer, 2011;10(0):147. 2011 [PMID: 22168338] (Flow,, Human) Flow Human
Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011;25(23):2465-79. 2011 [PMID: 22156207] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Naudin C, Joulin-Giet A, Couetdic G, Plesiat P, Szymanska A, Gorna E, Gauthier F, Kasprzykowski F, Lecaille F, Lalmanach G Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis. PLoS ONE, 2011;6(9):e25577. 2011 [PMID: 21980493] (WB,, Human) WB Human
Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010;56(0):1320. 2010 [PMID: 20530731] (Luminex Development,, Human) Luminex Development Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol CTSS
Uniprot