Cathepsin S Antibody [Unconjugated]


Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at more

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Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N, ELISA(Cap)

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Cathepsin S Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Accession # P25774
Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 0.2-1.8 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human Cathepsin S Antibody (Catalog # AF1183)
ELISA Detection: Human Cathepsin S Biotinylated Antibody (Catalog # BAF1183)
Standard: Recombinant Human Cathepsin S (Catalog # 1183-CY)
Read Publications using
AF1183 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin S Antibody [Unconjugated]

  • Cathepsin S
  • CTSS
  • EC 3.4.22
  • EC
  • FLJ50259
  • MGC3886


Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1-16), a pro region (residues 17-114), and the mature enzyme (residues 115-331) (3-5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages, and other phagocytic cells.

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin S Antibody (AF1183)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-P, Luminex Development, Western Blot.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF1183 Applications Species
D Bararia, JA Hildebrand, S Stolz, S Haebe, S Alig, CP Trevisani, F Osorio-Bar, MD Bartoschek, M Mentz, A Pastore, E Gaitzsch, M Heide, V Jurinovic, K Rautter, J Gunawardan, MB Sabdia, M Szczepanow, J Richter, W Klapper, A Louissaint, C Ludwig, S Bultmann, H Leonhardt, S Eustermann, KP Hopfner, W Hiddemann, M von Bergwe, C Steidl, R Kridel, JWD Tobin, MK Gandhi, DM Weinstock, M Schmidt-Su, MB Sárosi, M Rudelius, V Passerini, J Mautner, O Weigert Cathepsin S Alterations Induce a Tumor-Promoting Immune Microenvironment in Follicular Lymphoma Cell Rep, 2020;31(5):107522. 2020 [PMID: 32330423] (Western Blot, Human) Western Blot Human
The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity PLoS ONE, 2016;11(8):e0161573. 2016 [PMID: 27561012] (Western Blot, Chicken) Western Blot Chicken
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors Sci Rep, 2016;6(0):29256. 2016 [PMID: 27387133] (Western Blot, Human) Western Blot Human
Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer Oncotarget, 2016;7(19):28124-38. 2016 [PMID: 27058412] (IHC-P, Human) IHC-P Human
Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases J. Biol. Chem., 2016;291(16):8486-99. 2016 [PMID: 26884336] (Western Blot, Human) Western Blot Human
Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE, 2015;10(4):e0120348. 2015 [PMID: 25927437] (IHC, Mouse) IHC Mouse
Barry , Zachary, Platt , Manu O Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation. J Biol Chem, 2012;287(33):27723-30. 2012 [PMID: 22730330] (Western Blot, Human) Western Blot Human
Kwok HF, Buick RJ, Kuehn D Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity. Mol. Cancer, 2011;10(0):147. 2011 [PMID: 22168338] (Flow Cytometry, Human) Flow Cytometry Human
Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011;25(23):2465-79. 2011 [PMID: 22156207] (IHC-P, Human) IHC-P Human
Naudin C, Joulin-Giet A, Couetdic G, Plesiat P, Szymanska A, Gorna E, Gauthier F, Kasprzykowski F, Lecaille F, Lalmanach G Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis. PLoS ONE, 2011;6(9):e25577. 2011 [PMID: 21980493] (Western Blot, Human) Western Blot Human
Show All 14 Publications.

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Gene Symbol CTSS