DYNC1LI1 Antibody - BSA Free

Western Blot: DYNC1LI1 Antibody [NBP2-14935] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE; antibody diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: DYNC1LI1 Antibody [NBP2-14935] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: DYNC1LI1 Antibody [NBP2-14935] - Rat intestine. DYNC1LI1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: DYNC1LI1 Antibody [NBP2-14935] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunoprecipitation: DYNC1LI1 Antibody [NBP2-14935] - Immunoprecipitation of DYNC1LI1 protein from 293T whole cell extracts using 5 ug of DYNC1LI1 antibody. Western blot analysis was performed using DYNC1LI1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DYNC1LI1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DYNC1LI1. The exact sequence is proprietary.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DYNC1LI1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Bovine (86%), Chicken (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DYNC1LI1 Antibody - BSA Free

• cytoplasmic dynein 1 light intermediate chain 1
• DNCLI1
• dynein, cytoplasmic 1, light intermediate chain 1
• LIC1

Background

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DYNC1LI1 Antibody (NBP2-14935)(1)

Publication using NBP2-14935

• Cason SE, Carman PJ, Van Duyne C et al. Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway Journal of Cell Biology 2021-07-05 [PMID: 34014261]

Bioinformatics

• Gene Symbol: DYNC1LI1
• Uniprot:
  • Q9Y6G9(Human)