Dynactin Subunit 1/DCTN1 Antibody

Images

 
Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dynactin Subunit 1/DCTN1 Antibody Summary

Immunogen
Peptide with sequence C-QEQLHQLHSRLIS corresponding to C-Terminus according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1.
Localization
Cytoplasmic
Specificity
This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
ICC: An anonymous customer has reported positive results on mouse embryonic fibroblasts (MEF cells).
WB: Approx. 150 kDa band observed in A549 and in human testis lysates (calculated MW of 142 kDa band according to NP_004073.2). An anonymous customer has reported positive results on mouse embryonic fibroblasts (MEF cells). An anonymous customer has reported positive results on human HeLa cells.
IHC-P: Human cerebellum shows pixulate staining of cytoplasm in neuronal cells. Human testis shows cytoplasm staining in cells of the seminiferous tubules. An anonymous customer has reported positive results in on human HeLa cells.
Theoretical MW
142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Dynactin Subunit 1/DCTN1 Lysate (NBL1-09749)
Publications
Read Publications using
NB100-1110 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dynactin Subunit 1/DCTN1 Antibody

  • DAP-150
  • DCTN1
  • DP-150
  • dynactin 1 (p150, Glued (Drosophila) homolog)
  • Dynactin 1
  • Dynactin Subunit 1
  • glued homolog, Drosophila)
  • HMN7B
  • p135
  • p150 Glued (Drosophila) homolog
  • p150-glued

Background

The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
AF5414
Species: Hu
Applications: WB, Simple Western
NB500-207
Species: Hu
Applications: WB, ICC/IF, IP
NBP2-33855
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-76788
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-68205
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-30463
Species: Hu, Mu, Ze
Applications: WB, ICC/IF, IP
NBP1-80903
Species: Hu
Applications: IHC, IHC-P
NB100-56618
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-94712
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF1436
Species: Mu
Applications: WB
NB120-11433
Species: Hu, Mu, Rt, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
H00023435-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-45808
Species: Hu
Applications: IHC
NBP1-18878
Species: Hu
Applications: WB, IHC-P, IP
NBP2-32972
Species: Hu, Mu, Rt, Ch, Pm, Sh
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB100-74648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-02576
Species: Hu, Rt, Mk
Applications: WB, Flow, ICC/IF
NBP2-22169
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-1110
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110)(2)

We have publications tested in 1 confirmed species: Rabbit.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Rabbit
(1)
All Species

Reviews for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110) (0)

There are no reviews for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Dynactin Subunit 1/DCTN1 Products

Bioinformatics Tool for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110)

Discover related pathways, diseases and genes to Dynactin Subunit 1/DCTN1 Antibody (NB100-1110). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110)

Discover more about diseases related to Dynactin Subunit 1/DCTN1 Antibody (NB100-1110).
 

Pathways for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110)

View related products by pathway.

PTMs for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110)

Learn more about PTMs related to Dynactin Subunit 1/DCTN1 Antibody (NB100-1110).
 

Research Areas for Dynactin Subunit 1/DCTN1 Antibody (NB100-1110)

Find related products by research area.

Blogs on Dynactin Subunit 1/DCTN1

There are no specific blogs for Dynactin Subunit 1/DCTN1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Dynactin Subunit 1/DCTN1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DCTN1
Entrez
Uniprot