Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
ICC: An anonymous customer has reported positive results on mouse embryonic fibroblasts (MEF cells). WB: Approx. 150 kDa band observed in A549 and in human testis lysates (calculated MW of 142 kDa band according to NP_004073.2). An anonymous customer has reported positive results on mouse embryonic fibroblasts (MEF cells). An anonymous customer has reported positive results on human HeLa cells. IHC-P: Human cerebellum shows pixulate staining of cytoplasm in neuronal cells. Human testis shows cytoplasm staining in cells of the seminiferous tubules. An anonymous customer has reported positive results in on human HeLa cells. Use in Proximity Ligation Assay reported in scientific literature (PMID: 30354839).
142 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rabbit reactivity reported in scientific literature (PMID: 19136602).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Dynactin Subunit 1/DCTN1 Antibody
dynactin 1 (p150, Glued (Drosophila) homolog)
Dynactin Subunit 1
glued homolog, Drosophila)
p150 Glued (Drosophila) homolog
The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.