IER3 Antibody - BSA Free

Western Blot: IER3 Antibody [NBP1-76802] - Analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 ug/mL..

Immunohistochemistry: IER3 Antibody - BSA Free [NBP1-76802] - Immunohistochemistry of IER3 in human liver tissue with IER3 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: IER3 Antibody - BSA Free [NBP1-76802] - Immunofluorescence of IER3 in Human Brain cells with IER3 antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IER3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IER3 Antibody - BSA Free (NBP1-76802) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-IER3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 24 amino acid synthetic peptide near the center of human IEX1. The immunogen is located within amino acids 40 - 90 of IEX-1. Amino Acid Squence: APAGRPSASRGHRKRSRRVLYPR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IER3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IER3 Peptide (NBP1-76802PEP)

Reactivity Notes

Rat reactivity reported in (PMID: 22417305).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IER3 Antibody - BSA Free

• DIF-2
• DIF2GLY96
• Differentiation-dependent gene 2 protein
• expressed in pancreatic carcinoma
• gly96, mouse, homolog of
• IEX-1
• IEX1death protein
• IEX-1L
• Immediate early protein GLY96
• Immediate early response 3 protein
• immediate early response 3
• immediately early gene X-1
• PRG1PACAP-responsive gene 1 protein
• Protein DIF-2
• Protein PRG1
• radiation-inducible immediate-early gene IEX-1

Background

IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-alpha. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-kappaB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IER3 Antibody (NBP1-76802)(2)

Publications using NBP1-76802

• Jordan KR, Sikora MJ, Slansky JE et al. The Capacity of the Ovarian Cancer Tumor Microenvironment to Integrate Inflammation Signaling Conveys a Shorter Disease-free Interval Clinical Cancer Research 2020-12-01 [PMID: 32928797] (Immunohistochemistry, Rat)
• Akilov OE, Wu MX, Ustyugova IV et al. Resistance of Sezary cells to TNF-alpha-induced apoptosis is mediated in part by a loss of TNFR1 and a high level of the IER3 expression Exp Dermatol 2012-04-01 [PMID: 22417305] (IF/IHC, Rat)

Review for IER3 Antibody (NBP1-76802) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer IHC 07/05/2012

Summary

• Application: Immunohistochemistry

Bioinformatics

• Gene Symbol: IER3
• Entrez:
  • 8870(Human)
  • 15937(Mouse)
• Uniprot:
  • P46695(Human)