IER3 Antibody

Images

 
Western Blot: IER3 Antibody [NBP1-76802] - Analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 ug/mL..
Immunocytochemistry/ Immunofluorescence: IER3 Antibody [NBP1-76802] - Human Brain cells with IEX-1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: IER3 Antibody [NBP1-76802] - Human liver tissue with IEX-1 antibody at 2.5 ug/mL

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IER3 Antibody Summary

Immunogen
Antibody was raised against a 24 amino acid synthetic peptide near the center of human IEX1. The immunogen is located within amino acids 40 - 90 of IEX-1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IER3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Reviewed Applications
Read 1 Review rated 5
using
NBP1-76802 in the following applications:

Publications
Read Publication using
NBP1-76802 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Rat reactivity reported in (PMID: 22417305).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for IER3 Antibody

  • DIF-2
  • DIF2GLY96
  • Differentiation-dependent gene 2 protein
  • expressed in pancreatic carcinoma
  • gly96, mouse, homolog of
  • IEX-1
  • IEX1death protein
  • IEX-1L
  • Immediate early protein GLY96
  • Immediate early response 3 protein
  • immediate early response 3
  • immediately early gene X-1
  • PRG1PACAP-responsive gene 1 protein
  • Protein DIF-2
  • Protein PRG1
  • radiation-inducible immediate-early gene IEX-1

Background

IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-alpha. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-kappaB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IER3 Antibody (NBP1-76802)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Filter By Species
Rat
(1)
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Review for IER3 Antibody (NBP1-76802) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP1-76802:
Filter by Applications
IHC
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry IER3 NBP1-76802
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reviewed by:
Anonymous
IHC 07/05/2012
View

Summary

ApplicationImmunohistochemistry
Pub Med ID22417305
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IER3 Antibody (NBP1-76802) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/05/2012
Application: IHC
Species:

Bioinformatics

Gene Symbol IER3
Entrez
Uniprot