DMBT1/GP340 Antibody (548012) [CoraFluor™ 1]

• Reactivity: Mu
• Applications: WB
• Clone: 548012
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

DMBT1/GP340 Antibody (548012) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E.coli-derived recombinant mouse DMBT1
  Trp215-Gly420
  Accession # Q60997
• Specificity: Detects mouse DMBT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD163 or recombinant human SRCRB4D is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: DMBT1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for DMBT1/GP340 Antibody (548012) [CoraFluor™ 1]

• CRP-ductin
• deleted in malignant brain tumors 1 protein
• deleted in malignant brain tumors 1
• DMBT1
• Ebnerin
• FLJ61058
• Glycoprotein 340
• GP340
• gp-340
• GP340muclin
• Hensin
• MGC164738
• Muclin
• SAG
• Salivary agglutinin
• Surfactant pulmonary-associated D-binding protein
• Vomeroglandin

Background

Glycoprotein-340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp-340 has been shown to bind SP-D in a calcium dependent manner through a protein-protein interaction. Like SP-D, gp-340 is mainly found in lung tissue and immunohistochemistry revealed high expression in alveolar macrophages (1). An identical protein is present in saliva (salivary agglutinin) and both forms are encoded by the gene DMBT1 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DMBT1