DMBT1/GP340 Antibody (1G4) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DMBT1/GP340 Antibody (1G4) - BSA Free Summary

• Immunogen: Native gp-340 purified from bronchoalveolar lavage fluid from patients with pulmonary alveolar proteinosis.
• Epitope: Protein chain
• Specificity: Clone 1G4 Reacts with gp-340 and salivary agglutinin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DMBT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-23576 can be used in ELISA. (2) WB: In Western blotting after SDS-PAGE NBP2-23576 reacts strongly with GP-340 in non-reduced forms only. A dilution guideline of 1/2000 has proved successful. (1, 2, 3, 4) IHC: NBP2-23576 can be used in IHC. (1, 2)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for DMBT1/GP340 Antibody (1G4) - BSA Free

• CRP-ductin
• deleted in malignant brain tumors 1 protein
• deleted in malignant brain tumors 1
• DMBT1
• Ebnerin
• FLJ61058
• Glycoprotein 340
• GP340
• gp-340
• GP340muclin
• Hensin
• MGC164738
• Muclin
• SAG
• Salivary agglutinin
• Surfactant pulmonary-associated D-binding protein
• Vomeroglandin

Background

Glycoprotein (gp)-340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp-340 has been shown to bind SP-D in a calcium-dependent manner through a protein-protein interaction Like SP-D, gp-340 is found in the lungs and immunohistochemistry shows high expression in alveolar macrophages An identical protein, salivary agglutinin, is present in saliva (2-4) and both gp-340 and salivary agglutinin are encoded by the gene DMBT1 (5-7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DMBT1/GP340 Antibody (NBP2-23576)(2)

Publications using NBP2-23576

• Reichhardt MP, Messing M, Andersson S et al. Intestinal SALSA/dmbt1 levels are decreased in prematurely born infants Scand J Immunol 2020-10-12 [PMID: 33047342]
• Reichhardt MP, Loimaranta V, Lea SM, Johnson S Structures of SALSA/DMBT1 SRCR domains reveal the conserved ligand-binding mechanism of the ancient SRCR fold Life Sci Alliance 2020-04-01 [PMID: 32098784]

Bioinformatics

• Gene Symbol: DMBT1