Human SP-D Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human SP-D Quantikine ELISA Kit Summary

• Background: The Quantikine Human SP-D Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human SP-D in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SP-D and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SP-D showed linear curves that were parallel to the standard curves obtained using the Quant...ikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring SP-D.
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• Additional Information: Quantikine Quickit version available - results in 90 minutes (QK1920).
• Specificity: Natural and recombinant human SP-D
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: SFTPD

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human SP-D Quantikine ELISA Kit

• COLEC7collectin-7
• Collectin 7
• Collectin-7
• Lung surfactant protein D
• PSPD
• PSP-D
• SFTP4
• SFTP4pulmonary surfactant-associated protein D
• SFTPD
• SPD
• SP-D
• SP-Dpulmonary surfactant apoprotein
• surfactant protein D
• surfactant, pulmonary-associated protein D
• surfactant-associated protein, pulmonary 4

Background

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for SP-D (DSFPD0)(11)

Publications using DSFPD0

• Zhu, L;Wu, S;Zong, C;Chen, Y;Sun, C;Zhang, Y;Zhang, X;Shu, X;Lu, X;Wang, G;Peng, Q; A serum biomarker-based index for predicting progressive pulmonary fibrosis in patients with anti-synthetase syndrome: the LOCCM index Respiratory research 2025-11-25 [PMID: 41291658] (Human)
• Lesur, O;Segal, ED;Rego, K;Mercat, A;Asfar, P;Chagnon, F; Process-Specific Blood Biomarkers and Outcomes in COVID-19 Versus Non-COVID-19 ARDS (APEL-COVID Study): A Prospective, Observational Cohort Study Journal of clinical medicine 2024-10-04 [PMID: 39407979] (Human)
• Zhu, X;Zhang, Q;Du, X;Jiang, Y;Niu, Y;Wei, Y;Zhang, Y;Chillrud, SN;Liang, D;Li, H;Chen, R;Kan, H;Cai, J; Respiratory Effects of Traffic-Related Air Pollution: A Randomized, Crossover Analysis of Lung Function, Airway Metabolome, and Biomarkers of Airway Injury Environmental health perspectives 2023-05-01 [PMID: 37141245] (Human)
• T Bukreieva, H Svitina, V Nikulina, A Vega, O Chybisov, I Shablii, A Ustymenko, P Nemtinov, G Lobyntseva, I Skrypkina, V Shablii Treatment of Acute Respiratory Distress Syndrome Caused by COVID-19 with Human Umbilical Cord Mesenchymal Stem Cells International Journal of Molecular Sciences, 2023-02-23;24(5):. 2023-02-23 [PMID: 36901868] (Human)
• A Olsson, S Gustavsen, IC Hasselbalc, AR Langkilde, F Sellebjerg, AB Oturai, HB Søndergaar Biomarkers of inflammation and epithelial barrier function in multiple sclerosis Mult Scler Relat Disord, 2020-09-19;46(0):102520. 2020-09-19 [PMID: 32980645] (Human)
• J Avouac, A Cauvet, A Steelandt, Y Shirai, M Elhai, M Kuwana, O Distler, Y Allanore Improving risk-stratification of rheumatoid arthritis patients for interstitial lung disease PLoS ONE, 2020-05-08;15(5):e0232978. 2020-05-08 [PMID: 32384128] (Human)
• LL Negrin, G Halat, S Kettner, M Gregori, R Ristl, S Hajdu, T Heinz Club cell protein 16 and cytokeratin fragment 21-1 as early predictors of pulmonary complications in polytraumatized patients with severe chest trauma PLoS ONE, 2017-04-05;12(4):e0175303. 2017-04-05 [PMID: 28380043] (Human)
• Engels G, van Klarenbosch J, Gu Y, van Oeveren W, de Vries A Intraoperative cell salvage during cardiac surgery is associated with reduced postoperative lung injury. Interact Cardiovasc Thorac Surg, 2015-12-23;22(3):298-304. 2015-12-23 [PMID: 26705299] (Human)
• Yamane H, Ochi N, Yamagishi T, Honda Y, Takeyama M, Takigawa N Circulating thymus and activation-regulated chemokine/CC chemokine ligand 17 is a strong candidate diagnostic marker for interstitial lung disease in patients with malignant tumors: a result from a pilot study. Ther Clin Risk Manag, 2015-06-17;11(0):949-59. 2015-06-17 [PMID: 26124663] (Human)
• Kamio K, Usuki J, Azuma A, Matsuda K, Ishii T, Inomata M, Hayashi H, Kokuho N, Fujita K, Saito Y, Miya T, Gemma A Nintedanib modulates surfactant protein-D expression in A549 human lung epithelial cells via the c-Jun N-terminal kinase-activator protein-1 pathway. Pulm Pharmacol Ther, 2015-04-02;32(0):29-36. 2015-04-02 [PMID: 25843005] (Human)
• McIntyre B, Alev C, Mechael R, Salci K, Lee J, Fiebig-Comyn A, Guezguez B, Wu Y, Sheng G, Bhatia M Expansive generation of functional airway epithelium from human embryonic stem cells. Stem Cells Transl Med, 2013-12-03;3(1):7-17. 2013-12-03 [PMID: 24300555] (Human)

FAQs for SP-D (DSFPD0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SFTPD