DHX36 Antibody - BSA Free

Western Blot: DHX36 Antibody [NB200-353] - Whole cell lysate from NIH3T3 (50ug), TCKM (50ug) and Renca (50ug) cells. Antibodies: Affinity purified rabbit antiDHX36 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: DHX36 Antibody [NB200-353] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DHX36 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: DHX36 Antibody [NB200-353] - Detection of Human DHX36 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug) and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody NB200-353 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: DHX36 Antibody [NB200-353] - Detection of human DHX36 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody NB200-353 (lot NB200-353-2) used for IP at 6 ug/mg lysate. DHX36 was also immunoprecipitated by a previous lot (NB200-353-1) of this antibody. For blotting immunoprecipitated DHX36, NB200-353 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DHX36 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DHX36 Antibody - BSA Free (NB200-353) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-DHX36 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 950 and the C-terminus (residue 1008) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 36 using the numbering given in entry NP_065916.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHX36
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:5000-1:15000
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23651854).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DHX36 Antibody - BSA Free

• DDX36
• DEAH (Asp-Glu-Ala-His) box polypeptide 36
• DEAH box protein 36
• EC 3.6.1
• EC 3.6.4.13
• G4 resolvase-1
• KIAA1488G4R1
• MLEL1RHAUDEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36
• MLE-like protein 1
• probable ATP-dependent RNA helicase DHX36
• RNA helicase associated with AU-rich element ARE

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DHX36 Antibody (NB200-353)(3)

Publications using NB200-353

• Bicker S, Khudayberdiev S, Weib K et al. The DEAH-box helicase DHX36 mediates dendritic localization of the neuronal precursor-microRNA-134. Genes Dev 2013-05-01 [PMID: 23651854] (IP, Rat)
• Naji S, Ambrus G, Cimerman?i? P et al. Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol Cell Proteomics 2012-04-01 [PMID: 22174317]
• Kim T, Pazhoor S, Bao M et al. Aspartate-glutamate-alanine-histidine box motif (DEAH)/RNA helicase A helicases sense microbial DNA in human plasmacytoid dendritic cells. Proc Natl Acad Sci U S A 2010-08-01 [PMID: 20696886]

Bioinformatics

• Gene Symbol: DHX36
• Entrez:
  • 170506(Human)
  • 72162(Mouse)
• Uniprot:
  • Q9H2U1(Human)