ZIC1 Antibody

Images

 
Western Blot: Zic1 Antibody [NB600-488] - WB analysis of Zic1 in overexpression lysate.
Immunohistochemistry: Zic1 Antibody [NB600-488] - IHC analysis of Zic1 in mouse kidney using DAB with hematoxylin counterstain.
Western Blot: Zic1 Antibody [NB600-488] - Western blot analysis of Zic1 in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ZIC1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human Zic1 protein (between residues 20-75) [UniProt Q15915]
Localization
Nuclear.
Marker
Ectoderm Marker
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZIC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This Zic1 antibody is useful for Western Blot and Immunohistochemisty-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemisty-frozen sections was reported in the scientific literature (PMID: 23862012).
Publications
Read Publications using
NB600-488 in the following applications:

Reactivity Notes

Human and mouse. Predicted to react with rat based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZIC1 Antibody

  • Zic family member 1 (odd-paired homolog, Drosophila)
  • ZIC1
  • ZICZic family member 1 (odd-paired Drosophila homolog)
  • Zinc finger protein 201
  • Zinc finger protein of the cerebellum 1
  • ZNF201
  • ZNF201zinc finger protein ZIC 1

Background

Zinc finger protein ZIC 1 (ZIC1) is a member of the ZIC family of C2H2-type zinc finger proteins. These proteins are particularly important during embryonic development. ZIC1 is a transcriptional activator via interaction with GLI1, GLI2, and GLI3. ZIC1 facilitates development and maturation of the cerebellum and spinal cord during organogenesis of the CNS. Within the pontine gray nucleus (PGN) ZIC1 plays a role in spatial distribution of mossy fiber neurons. The interaction between Zic and Gli family proteins are significant for molecular antagonistic and synergistic factors in development. Zic has been shown involvement in modulation of the hedgehog signaling pathway. ZIC1 may be found in the nucleus or in the cytoplasm in the presence of MDFIC overexpression. This protein is mainly found within the CNS and has high levels of expression in the cerebellum. In cancerous tissue, the ZIC1 protein is often found in medulloblastomas. ZIC1 has been used as aneuroepithelial marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZIC1 Antibody (NB600-488)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZIC1 Antibody (NB600-488). (Showing 1 - 1 of 1 FAQs).

  1. Has this antibody been recently qualified for use in chromatin immunoprecitiation?
    • NB600-488 has not been validated and can therefore not be guaranteed for ChIP. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-488AF405
Alexa Fluor 488 Labeled NB600-488AF488
Alexa Fluor 647 Labeled NB600-488AF647
Alexa Fluor 700 Labeled NB600-488AF700
Biotin Labeled NB600-488B
DyLight 405 Labeled NB600-488V
DyLight 488 Labeled NB600-488G
DyLight 550 Labeled NB600-488R
DyLight 650 Labeled NB600-488C
FITC Labeled NB600-488F
HRP Labeled NB600-488H

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Bioinformatics

Gene Symbol ZIC1
Entrez
Uniprot