ZIC1 Antibody


Western Blot: ZIC1 Antibody [NB600-488] - WB analysis of Zic1 in overexpression lysate.
Immunohistochemistry-Paraffin: ZIC1 Antibody [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants. Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) and IB4 (blue) highlight ...read more
Western Blot: ZIC1 Antibody [NB600-488] - Western blot analysis of Zic1 in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the ...read more
Immunohistochemistry: ZIC1 Antibody [NB600-488] - IHC analysis of Zic1 in mouse kidney using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: ZIC1 Antibody [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation and density in the cerebral vasculature. Zic1 (green), BrdU (red), and IB4 (blue) ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

ZIC1 Antibody Summary

A synthetic peptide made to an internal portion of the human Zic1 protein (between residues 20-75) [UniProt Q15915]
Ectoderm Marker
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This Zic1 antibody is useful for Western Blot and Immunohistochemisty-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemisty-frozen sections was reported in the scientific literature (PMID: 23862012).
Read Publications using
NB600-488 in the following applications:

Reactivity Notes

Human and mouse. Predicted to react with rat based on 100% sequence homology.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.01% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for ZIC1 Antibody

  • Zic family member 1 (odd-paired homolog, Drosophila)
  • ZIC1
  • ZICZic family member 1 (odd-paired Drosophila homolog)
  • Zinc finger protein 201
  • Zinc finger protein of the cerebellum 1
  • ZNF201
  • ZNF201zinc finger protein ZIC 1


Zinc finger protein ZIC 1 (ZIC1) is a member of the ZIC family of C2H2-type zinc finger proteins. These proteins are particularly important during embryonic development. ZIC1 is a transcriptional activator via interaction with GLI1, GLI2, and GLI3. ZIC1 facilitates development and maturation of the cerebellum and spinal cord during organogenesis of the CNS. Within the pontine gray nucleus (PGN) ZIC1 plays a role in spatial distribution of mossy fiber neurons. The interaction between Zic and Gli family proteins are significant for molecular antagonistic and synergistic factors in development. Zic has been shown involvement in modulation of the hedgehog signaling pathway. ZIC1 may be found in the nucleus or in the cytoplasm in the presence of MDFIC overexpression. This protein is mainly found within the CNS and has high levels of expression in the cerebellum. In cancerous tissue, the ZIC1 protein is often found in medulloblastomas. ZIC1 has been used as aneuroepithelial marker.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZIC1 Antibody (NB600-488)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZIC1 Antibody (NB600-488). (Showing 1 - 1 of 1 FAQs).

  1. Has this antibody been recently qualified for use in chromatin immunoprecitiation?
    • NB600-488 has not been validated and can therefore not be guaranteed for ChIP. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-488AF350
Alexa Fluor 405 NB600-488AF405
Alexa Fluor 488 NB600-488AF488
Alexa Fluor 532 NB600-488AF532
Alexa Fluor 594 NB600-488AF594
Alexa Fluor 647 NB600-488AF647
Alexa Fluor 700 NB600-488AF700
Alexa Fluor 750 NB600-488AF750
Biotin NB600-488B
DyLight 350 NB600-488UV
DyLight 405 NB600-488V
DyLight 488 NB600-488G
DyLight 550 NB600-488R
DyLight 594 NB600-488DL594
DyLight 650 NB600-488C
DyLight 680 NB600-488FR
DyLight 755 NB600-488IR
FITC NB600-488F
HRP NB600-488H
Janelia Fluor 549 NB600-488JF549
Janelia Fluor 646 NB600-488JF646

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Gene Symbol ZIC1