DFF45/ICAD Antibody - BSA Free

Western Blot: DFF45/ICAD Antibody [NBP3-03452] - Western blot analysis of extracts of various cell lines, using DFF45/ICAD antibody (NBP3-03452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunocytochemistry/Immunofluorescence: DFF45/ICAD Antibody [NBP3-03452] - Immunofluorescence analysis of HeLa cells using DFF45/ICAD antibody (NBP3-03452). Blue: DAPI for nuclear staining.

Western Blot: DFF45/ICAD Antibody [NBP3-03452] - Western blot analysis of extracts from normal (control) and DFF45/ICAD knockout (KO) HeLa cells, using DFF45/ICAD antibody (NBP3-03452) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DFF45/ICAD Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit DFF45/ICAD Antibody - BSA Free (NBP3-03452) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFF45/ICAD (NP_004392.1). MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DFFA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DFF45/ICAD Antibody - BSA Free

• DFF1
• DFF1DNA fragmentation factor 45 kDa subunit
• DFF45
• DFF45DNA fragmentation factor subunit alpha
• DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
• DFFA
• DNA fragmentation factor, 45kDa, alpha polypeptide
• ICAD
• Inhibitor of CAD

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DFFA