DFF45/ICAD Antibody


Western Blot: DFF45/ICAD Antibody [NBP1-77034] - Analysis of HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with ICAD antibody at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody [NBP1-77034] - Staining of HeLa cells with ICAD antibody at 5 ug/ml.
Western Blot: DFF45/ICAD Antibody [NBP1-77034] - Western blot analysis of 50 ug of total cell lysate from HeLa cells with anti-DFF45 (CT) at 1:2000 dilution.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1 mg/ml

Order Details

DFF45/ICAD Antibody Summary

Antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45.
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Ion exchange chromatography


Application Notes
DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blotat 1:1000 to 1:2000 dilution and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells.
Theoretical MW
45 kDa
Control Peptide
DFF45/ICAD Peptide (NBP1-77034PEP)
Read Publication using NBP1-77034.

Alternate Names for DFF45/ICAD Antibody

  • DFF1
  • DFF1DNA fragmentation factor 45 kDa subunit
  • DFF45DNA fragmentation factor subunit alpha
  • DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
  • DFFA
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • ICAD
  • Inhibitor of CAD


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DFF45/ICAD Antibody (NBP1-77034)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol DFFA

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