DDX5 Antibody

Images

 
Western Blot: DDX5 Antibody [NBP1-30944] - Human lymphocytes. Antibody 1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA. This image was submitted via customer ...read more
Immunocytochemistry/ Immunofluorescence: DDX5 Antibody [NBP1-30944] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-30944] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.
Western Blot: DDX5 Antibody [NBP1-30944] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE DDX5 antibody, antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DDX5 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDX5. The exact sequence is proprietary.
Localization
Nucleus, nucleolus
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Primate (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3
using
NBP1-30944 in the following applications:

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for DDX5 Antibody

  • B-cell CLL/lymphoma 7 protein family member C
  • B-cell CLL/lymphoma 7C
  • DDX5
  • DEAD/H
  • G17P1
  • HELR
  • HLR1
  • HUMP68

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX5 Antibody (NBP1-30944) (0)

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Reviews for DDX5 Antibody (NBP1-30944) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-30944:
Filter by Applications
WB
(1)
ICC
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot DDX5 NBP1-30944
Enlarge
reviewed by:
Anonymous
WB Human 03/13/2018
View

Summary

ApplicationWestern Blot
Sample TestedLymphocytes
SpeciesHuman
Lot369274

Comments

CommentsBIOSCARF
Immunocytochemistry DDX5 NBP1-30944
Enlarge
reviewed by:
Anonymous
ICC Human 01/11/2018
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
Lot369274

Comments

Comments1:500 in 1X PBS/1% BSA/0.3% Trito X-100, 16 hours, 4C.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DDX5 Antibody (NBP1-30944) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DDX5 Antibody (NBP1-30944)

Discover related pathways, diseases and genes to DDX5 Antibody (NBP1-30944). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for DDX5 Antibody (NBP1-30944)

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Recent Reviews

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5
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1
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Anonymous
03/13/2018
Application: WB
Species: Human

Anonymous
01/11/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol DDX5
Entrez