DDX5 Antibody - BSA Free

Western Blot: DDX5 Antibody [NBP1-30944] - Human lymphocytes. Antibody 1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA. This image was submitted via customer review.

Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP1-30944] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-30944] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.

Western Blot: DDX5 Antibody [NBP1-30944] - 30 ug of whole cell lysate, H1299 7. 5% SDS-PAGE DDX5. Antibody at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDX5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDX5 Antibody - BSA Free (NBP1-30944) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDX5. The exact sequence is proprietary.
• Localization: Nucleus , nucleolus
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Primate (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DDX5 Antibody - BSA Free

• B-cell CLL/lymphoma 7 protein family member C
• B-cell CLL/lymphoma 7C
• DDX5
• DEAD/H
• G17P1
• HELR
• HLR1
• HUMP68

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Reviews for DDX5 Antibody (NBP1-30944) (3)

Average Rating: 3.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer IP Human 01/02/2019

Summary

• Application: Immunoprecipitation
• Sample Tested: B-cell
• Species: Human
• Lot: 369274

Comments

• Comments: 10ug antibody used with 500ug human B-cell whole cell lysate. L1: 40ug input, L2: IgG, L3: IP (10ul of 50ul elution)

reviewed by: Verified Customer WB Human 03/13/2018

Summary

• Application: Western Blot
• Sample Tested: Lymphocytes
• Species: Human
• Lot: 369274

Comments

• Comments: BIOSCARF

reviewed by: Verified Customer ICC Human 01/11/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: Human
• Lot: 369274

Comments

• Comments: 1:500 in 1X PBS/1% BSA/0.3% Trito X-100, 16 hours, 4C.

Bioinformatics

• Gene Symbol: DDX5