DNA polymerase alpha Antibody

Images

 
Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit ...read more
Proximity Ligation Assay: DNA polymerase alpha Antibody [NBP1-47257] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human POLA1 (NBP1-47257). Image merged from DAPI ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

DNA polymerase alpha Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1175 and 1225 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLA1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200 - 1:4000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA polymerase alpha Antibody

  • DNA polymerase alpha 1 catalytic subunit
  • DNA polymerase alpha catalytic subunit p180
  • DNA polymerase alpha catalytic subunit
  • DNA polymerase alpha p180 subunit
  • EC 2.7.7.7
  • p180
  • POLADKFZp686K1672
  • polymerase (DNA directed), alpha 1
  • polymerase (DNA directed), alpha 1, catalytic subunit
  • polymerase (DNA directed), alpha
  • polymerase (DNA-directed), alpha (70kD)

Background

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and episilon are involved in DNA replication and repair. DNA pol delta and DNA pol episilon are multisubunit enzymes, with DNA pol delta consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLA1
Entrez
Uniprot