DDX5 Antibodies
We offer DDX5 Antibodies for use in common research applications: Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each DDX5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DDX5 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the DDX5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DDX5 polyclonal antibodies and browse our DDX5 monoclonal antibody catalog.
Alternate Names for DDX5 Antibodies
anti-DDX5 antibody, anti-B-cell CLL/lymphoma 7 protein family member C antibody, anti-B-cell CLL/lymphoma 7C antibody
11 Results for "ddx5" in Primary Antibodies
![Western Blot: DDX5 Antibody [NB200-351] - Detection of Human and Mouse DDX5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody NB200-351 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB200-351-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: DDX5 Antibody [NB200-351] - Detection of Human and Mouse DDX5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody NB200-351 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: DDX5 Antibody [NB200-351] - Detection of human DDX5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DDX5 NB200-351 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0009.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DDX5 Antibody [NB200-351] - Detection of DDX5 in whole cell lysates from HeLa (5, 15, 50 ug), 293 (T; 50 ug), and MCF7 (M; 50 ug), and K562 (K; 50 ug) for WB (A) and HeLa (1 mg) for IP (B). Antibodies: NB 200-351 used at 0.04 ug/ml for WB (A & B) and at 3 ug/ml lysate for IP (B). DDX5 was also immonuprecipitated with NB 200-350 used at 3 ug/ml lysate. Detection: ECL 30 seconds.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB200-351-img0001.jpg)
![Immunohistochemistry: DDX5 Antibody [NB200-351] - ~ ~Sample: FFPE section of human breast carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0003.jpg)
![Immunohistochemistry: DDX5 Antibody [NB200-351] - ~~ Sample: FFPE section of mouse renal cell carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0004.jpg)
![Immunohistochemistry: DDX5 Antibody [NB200-351] - Detection of mouse DDX5 by immunohistochemistry. Sample: FFPE section of mouse skin. Antibody: Affinity purified rabbit anti-DDX5 NB200-351 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX5 Antibody [NB200-351] - Detection of human DDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX5 antibody NB200-351 (lot NB200-351-2) used for IP at 6 ug per reaction. DDX5 was also immunoprecipitated by a previous lot of this antibody (lot NB200-351-1) and another rabbit anti-DDX5 antibody. For blotting immunoprecipitated DDX5, NB200-351 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunoprecipitation-NB200-351-img0007.jpg)
![Western Blot: DDX5 Antibody (1K6X10) [NBP3-15371] - Western blot analysis of extracts of various cell lines, using DDX5 Rabbit mAb (NBP3-15371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Western-Blot-NBP3-15371-img0007.jpg "Western Blot: DDX5 Antibody (1K6X10) [NBP3-15371] - Western blot analysis of extracts of various cell lines, using DDX5 Rabbit mAb (NBP3-15371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunofluorescence analysis of NIH-3T3 cells using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunocytochemistry-Immunofluorescence-NBP3-15371-img0006.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunohistochemistry of paraffin-embedded mouse spinal cord using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunohistochemistry-Paraffin-NBP3-15371-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunofluorescence analysis of C6 cells using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunocytochemistry-Immunofluorescence-NBP3-15371-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunofluorescence analysis of HeLa cells using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunocytochemistry-Immunofluorescence-NBP3-15371-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunohistochemistry of paraffin-embedded rat ovary using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunohistochemistry-Paraffin-NBP3-15371-img0001.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunohistochemistry of paraffin-embedded human esophageal using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunohistochemistry-Paraffin-NBP3-15371-img0002.jpg)
![Immunoprecipitation: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunoprecipitation analysis of 600ug extracts of Mouse testis using 3ug DDX5 antibody (NBP3-15371). Western blot was performed from the immunoprecipitate using DDX5 (NBP3-15371) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunoprecipitation-NBP3-15371-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX5 Antibody (1K6X10) [DDX5] - Confocal imaging of Hep G2 cells using DDX5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517184541.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517213590.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517192961.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-5220251728497.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX5 Antibody (1K6X10) [DDX5] - Confocal imaging of C2C12 cells using DDX5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-5220251725190.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517152765.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517152748.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517184527.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX5 Antibody (1K6X10) [DDX5] - Confocal imaging of U-2 OS cells using DDX5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-5220251721351.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517172893.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517273533.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517273592.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517273531.jpg)
![Western Blot: DDX5 Antibody (1K6X10) [DDX5] - Western blot analysis of various lysates using DDX5 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517284924.jpg)
![Immunohistochemistry: DDX5 Antibody (1K6X10) [DDX5] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DDX5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15371_rabbit-ddx5-mab-1k6x10-52202517284915.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0017.jpg "Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.")
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0014.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0015.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0016.jpg)
![Western Blot: DDX5 Antibody [NBP1-30944] - Human lymphocytes. Antibody 1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA. This image was submitted via customer review.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP1-30944-img0006.jpg "Western Blot: DDX5 Antibody [NBP1-30944] - Human lymphocytes. Antibody 1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA. This image was submitted via customer review.")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP1-30944] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30944-img0003.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-30944] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-30944-img0004.jpg)
![Western Blot: DDX5 Antibody [NBP1-30944] - 30 ug of whole cell lysate, H1299 7. 5% SDS-PAGE DDX5. Antibody at 1:1000.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP1-30944-img0005.jpg)
![Western Blot: DDX5 Antibody [NB100-1410] - (0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB100-1410-img0006.jpg "Western Blot: DDX5 Antibody [NB100-1410] - (0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DDX5 Antibody [NB100-1410] - Paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained using a previous batch.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NB100-1410-img0004.jpg)
![Western Blot: DDX5 Antibody [NB100-1410] - DDX5 / p68 RNA helicase in Hela lysate using this antibody at 0.5 ug/ ml.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB100-1410-img0005.jpg)
![Immunohistochemistry: DDX5 Antibody (2C3) [NBP3-26627] - Image of DDX5 Antibody (2C3) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-26220241422520.jpg "Immunohistochemistry: DDX5 Antibody (2C3) [NBP3-26627] - Image of DDX5 Antibody (2C3) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: DDX5 Antibody (2C3) [NBP3-26627] - Positive Western Blot detected in: HepG2 whole cell lysate, HT-29 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue. All lanes: DDX5 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70, 61 kDa Observed band size: 70 kDa](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-282202411413612.jpg)
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (2C3) [NBP3-26627] - Staining of HepG2 Cells with DDX5 Antibody (2C3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-28220241323637.jpg)
![Flow Cytometry: DDX5 Antibody (2C3) [NBP3-26627] - Overlay histogram showing Hela cells stained with DDX5 Antibody (2C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-282202414183613.jpg)
![Western Blot: DDX5 Antibody [NBP2-98889] - Anti-DDX5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP2-98889-img0003.jpg "Western Blot: DDX5 Antibody [NBP2-98889] - Anti-DDX5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 70 kDa")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP2-98889] - Immunofluorescence staining of DDX5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DDX5 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98889-img0002.jpg)
![Immunoprecipitation: DDX5 Antibody [NBP2-98889] - DDX5 was immunoprecipitated using: Lane A: 0.5 mg PC-3 Whole Cell Lysate4 ul anti-DDX5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DDX5 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunoprecipitation-NBP2-98889-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP2-98889] - Immunofluorescence staining of DDX5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98889 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98889_rabbit-polyclonal-ddx5-antibody-235202514331595.png)
![Western Blot: DDX5 Antibody [NBP3-17654] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP3-17654-img0002.jpg "Western Blot: DDX5 Antibody [NBP3-17654] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP3-17654] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17654-img0001.jpg)