DDAH2 Antibody - BSA Free

Staining of human cell line A-431 shows localization to cytosol.

Immunohistochemistry-Paraffin: DDAH2 Antibody [NBP1-86092] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: DDAH2 Antibody [NBP1-86092] - Staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: DDAH2 Antibody [NBP1-86092] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: DDAH2 Antibody [NBP1-86092] - Staining of human small intestine shows very strong cytoplasmic positivity in glandular cells.

Analysis in human cell line EFO-21.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDAH2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDAH2 Antibody - BSA Free (NBP1-86092) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDAH2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  DDAH2 Protein (NBP1-86092PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DDAH2 Antibody - BSA Free

• DDAHII
• dimethylargininase-2
• dimethylarginine dimethylaminohydrolase 2G6a
• dimethylarginine dimethylaminohydrolase II
• EC 3.5.3.18
• G6a
• N(G)
• N(G)-dimethylarginine dimethylaminohydrolase 2
• NG30
• NG-dimethylarginine dimethylamino hydrolase homolog
• S-phase protein

Background

DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDAH2