MHC Class II Antibody (M5/114) - Azide and BSA Free

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Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
M5/114
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

MHC Class II Antibody (M5/114) - Azide and BSA Free Summary

Immunogen
Activated C57BL/6 mouse spleen cells
Specificity
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
HLA-DMA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Functional
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:2000
Application Notes
Functional Application: Blocking of T cell proliferative responses. This antibody is CyTOF ready.
Publications
Read Publications using
NBP2-21789 in the following applications:

  • 1 publication
  • IHC
    2 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34166814).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate buffered saline (PBS), pH 7.4
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for MHC Class II Antibody (M5/114) - Azide and BSA Free

  • class II histocompatibility antigen, M alpha chain
  • D6S222E
  • DMA
  • HLA class II histocompatibility antigen, DM alpha chain
  • major histocompatibility complex, class II, DM alpha
  • MHC class II antigen DMA
  • Really interesting new gene 6 protein
  • RING6HLADM

Background

MHC(major histocompatibility complex)class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MHC Class II Antibody (NBP2-21789)(3)

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Blogs on MHC Class II.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.


  Read full blog post.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required ...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-DMA