MHC Class II Antibody (M5/114) - Azide and BSA Free

Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.

• Reactivity: Mu, Rt
• Applications: WB, Flow, Func, IHC, IP, CyTOF-ready
• Clone: M5/114
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MHC Class II Antibody (M5/114) - Azide and BSA Free Summary

• Immunogen: Activated C57BL/6 mouse spleen cells
• Specificity: The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: HLA-DMA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Functional
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: Functional Application: Blocking of T cell proliferative responses. This antibody is CyTOF ready.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34166814).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for MHC Class II Antibody (M5/114) - Azide and BSA Free

• class II histocompatibility antigen, M alpha chain
• D6S222E
• DMA
• HLA class II histocompatibility antigen, DM alpha chain
• major histocompatibility complex, class II, DM alpha
• MHC class II antigen DMA
• Really interesting new gene 6 protein
• RING6HLADM

Background

MHC(major histocompatibility complex)class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MHC Class II Antibody (NBP2-21789)(6)

Publications using NBP2-21789

• Dharshika C, Gonzales J, Chow A et al. Stimulator of interferon genes (STING) expression in the enteric nervous system and contributions of glial STING in disease Neurogastroenterology & Motility 2023-02-27 (Immunohistochemistry)
• Dharshika C, Gonzales J, Chow A, Morales-Soto W et Al. Stimulator of interferon genes (STING) expression in the enteric nervous system and contributions of glial STING in disease Neurogastroenterol Motil 2023-06-13 [PMID: 37309618]
• Chow AK, Grubisic V,Gulbransen BD Enteric glia regulate lymphocyte activation via autophagy-mediated MHC-II expression Cellular and molecular gastroenterology and hepatology 2021-06-21 [PMID: 34166814] (IF/IHC, Rat)
• V Grubiši?, JL McClain, DE Fried, I Grants, P Rajasekhar, E Csizmadia, OA Ajijola, RE Watson, DP Poole, SC Robson, FL Christofi, BD Gulbransen Enteric Glia Modulate Macrophage Phenotype and Visceral Sensitivity following Inflammation Cell Rep, 2020-09-08;32(10):108100. 2020-09-08 [PMID: 32905782] (Flow Cytometry, Mouse)
• Brown I The antioxidant glutathione protects against enteric neuron death in situ, but its depletion is protective during colitis. Am J Physiol Gastrointest Liver Physiol 2018-01-01 [PMID: 28882823] (IF/IHC, Mouse)
• McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. 2002-03-15 [PMID: 11877283] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: HLA-DMA