DDAH2 Antibody (5M4Y4)

Western Blot: DDAH2 Antibody (5M4Y4) [NBP3-16345] - Western blot analysis of extracts of various cell lines, using DDAH2 Rabbit mAb (NBP3-16345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 5M4Y4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DDAH2 Antibody (5M4Y4) Summary

• Description: Novus Biologicals Rabbit DDAH2 Antibody (5M4Y4) (NBP3-16345) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDAH2 (O95865). MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEV
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDAH2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DDAH2 Antibody (5M4Y4)

• DDAHII
• dimethylargininase-2
• dimethylarginine dimethylaminohydrolase 2G6a
• dimethylarginine dimethylaminohydrolase II
• EC 3.5.3.18
• G6a
• N(G)
• N(G)-dimethylarginine dimethylaminohydrolase 2
• NG30
• NG-dimethylarginine dimethylamino hydrolase homolog
• S-phase protein

Background

DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDAH2