DC-SIGN/CD209 Antibody (LWC06) - BSA Free

Western Blot: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - CIRE/DC-SIGN-transfected (left) and untransfected control CHO lysates were loaded at 1x10^5 cells/lane, probed with 1 ug/mL of Anti-Mouse CD209 (DC-SIGN) Purified and revealed with Anti-Rat IgG HRP.

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the PE conjugate of NBP1-43328..

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the Allophycocyanin conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.125 ug of Rat IgG2a kappa Isotype Control APC (blue histogram) or 0.125 ug of Anti-Mouse CD209 (DC-SIGN) APC (purple histogram).

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the FITC conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.5 ug of Rat IgG2a kappa Isotype Control FITC (open histogram) or 0.5 ug of Anti-Mouse CD209 (DC-SIGN) FITC (filled histogram).

• Reactivity: Mu
• Applications: WB, Flow, IP
• Clone: LWC06
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

DC-SIGN/CD209 Antibody (LWC06) - BSA Free Summary

• Description: Novus Biologicals Rat DC-SIGN/CD209 Antibody (LWC06) - BSA Free (NBP1-43328) is a monoclonal antibody validated for use in WB, Flow and IP. Anti-DC-SIGN/CD209 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was CIRE.
• Marker: Dendritic Cell Marker
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD209
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This LWC06 antibody has been tested by immunoblotting and immunoprecipitation of CIRE in CIRE-transfected CHO cells. The reactivity of this antibody has been confirmed by immunoprecipitation of CIRE with LWC06 followed by immunoblotting with another CIRE-specific monoclonal antibody, 5H10 and, immunoprecipitation of CIRE with 5H10 followed by immunoblotting with LWC06.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for DC-SIGN/CD209 Antibody (LWC06) - BSA Free

• CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
• CD209 molecule
• CD209
• CDSIGNHIV gpl20-binding protein
• CLEC4L
• CLEC4LC-type lectin domain family 4 member L
• DCSIGN
• DC-SIGN
• DC-SIGN1
• DC-SIGN1C-type lectin domain family 4, member L
• DC-SIGNMGC129965
• Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca2+ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The LWC06 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DC-SIGN/CD209 Antibody (NBP1-43328)(1)

Publication using NBP1-43328

• Chen Y, Guo KM, Nagy T, Guo TL Chronic oral exposure to glycated whey proteins increases survival of aged male NOD mice with autoimmune prostatitis by regulating the gut microbiome and anti-inflammatory responses Food Funct 2019-12-12 [PMID: 31829366] (Mouse)

Bioinformatics

• Gene Symbol: CD209