DC-SIGN/CD209 Antibody (LWC06)


Western Blot: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - CIRE/DC-SIGN-transfected (left) and untransfected control CHO lysates were loaded at 1x10^5 cells/lane, probed with 1 ug/mL of Anti-Mouse CD209 (DC-SIGN) ...read more
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the PE conjugate of NBP1-43328..
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the Allophycocyanin conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.125 ug of Rat IgG2a kappa Isotype ...read more
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the FITC conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.5 ug of Rat IgG2a kappa Isotype Control FITC ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IP
0.5 mg/ml

Order Details

DC-SIGN/CD209 Antibody (LWC06) Summary

The immunogen for this antibody was CIRE.
Dendritic Cell Marker
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunoprecipitation 1:10-1:500
Application Notes
This LWC06 antibody has been tested by immunoblotting and immunoprecipitation of CIRE in CIRE-transfected CHO cells. The reactivity of this antibody has been confirmed by immunoprecipitation of CIRE with LWC06 followed by immunoblotting with another CIRE-specific monoclonal antibody, 5H10 and, immunoprecipitation of CIRE with 5H10 followed by immunoblotting with LWC06.

Alternate Names for DC-SIGN/CD209 Antibody (LWC06)

  • CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
  • CD209 molecule
  • CD209
  • CDSIGNHIV gpl20-binding protein
  • CLEC4L
  • CLEC4LC-type lectin domain family 4 member L
  • DC-SIGN1
  • DC-SIGN1C-type lectin domain family 4, member L
  • DC-SIGNMGC129965
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 1


The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca2+ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The LWC06 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-43010
Biotin Labeled NBP1-43566
FITC Labeled NBP1-43965
PE Labeled NBP1-43790

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Contact Information

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Gene Symbol CD209

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