Home » DC-SIGN/CD209 » DC-SIGN/CD209 Antibodies » DC-SIGN/CD209 Antibody (LWC06)

DC-SIGN/CD209 Antibody (LWC06)

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Western Blot: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - CIRE/DC-SIGN-transfected (left) and untransfected control CHO lysates were loaded at 1x10^5 cells/lane, probed with 1 ug/mL of Anti-Mouse CD209 (DC-SIGN) Purified and revealed with Anti-Rat IgG HRP.
Western Blot: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - CIRE/DC-SIGN-transfected (left) and untransfected control CHO lysates were loaded at 1x10^5 cells/lane, probed with 1 ug/mL of Anti-Mouse CD209 (DC-SIGN) ...read more
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the PE conjugate of NBP1-43328..
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the Allophycocyanin conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.125 ug of Rat IgG2a kappa Isotype ...read more
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the FITC conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.5 ug of Rat IgG2a kappa Isotype Control FITC ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IP
Clone
LWC06
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43328AF350
Alexa Fluor 405
NBP1-43328AF405
Alexa Fluor 488
NBP1-43328AF488
Alexa Fluor 532
NBP1-43328AF532
Alexa Fluor 594
NBP1-43328AF594
Alexa Fluor 647
NBP1-43328AF647
Alexa Fluor 700
NBP1-43328AF700
Alexa Fluor 750
NBP1-43328AF750
Allophycocyanin
NBP1-43328APC
Allophycocyanin/Cy7
NBP1-43328APCCY7
Biotin
NBP1-43328B
DyLight 350
NBP1-43328UV
DyLight 405
NBP1-43328V
DyLight 488
NBP1-43328G
DyLight 550
NBP1-43328R
DyLight 594
NBP1-43328DL594
DyLight 650
NBP1-43328C
DyLight 680
NBP1-43328FR
DyLight 755
NBP1-43328IR
FITC
NBP1-43328F
FITC
NBP1-43965-0.025mg
HRP
NBP1-43328H
Janelia Fluor 549
NBP1-43328JF549
Janelia Fluor 646
NBP1-43328JF646
PE
NBP1-43328PE
PE
NBP1-43790-0.1mg
PE
NBP1-43790-0.025mg
PE/Atto594
NBP1-43328PEATT594
PE/Cy5.5
NBP1-43328PECY55
PE/Cy7
NBP1-43328PECY7
PerCP
NBP1-43328PCP
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DC-SIGN/CD209 Antibody (LWC06) Summary

Immunogen
The immunogen for this antibody was CIRE.
Marker
Dendritic Cell Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD209
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunoprecipitation 1:10-1:500
Application Notes
This LWC06 antibody has been tested by immunoblotting and immunoprecipitation of CIRE in CIRE-transfected CHO cells. The reactivity of this antibody has been confirmed by immunoprecipitation of CIRE with LWC06 followed by immunoblotting with another CIRE-specific monoclonal antibody, 5H10 and, immunoprecipitation of CIRE with 5H10 followed by immunoblotting with LWC06.
Publications
Read Publication using NBP1-43328.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for DC-SIGN/CD209 Antibody (LWC06)

  • CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
  • CD209 molecule
  • CD209
  • CDSIGNHIV gpl20-binding protein
  • CLEC4L
  • CLEC4LC-type lectin domain family 4 member L
  • DCSIGN
  • DC-SIGN
  • DC-SIGN1
  • DC-SIGN1C-type lectin domain family 4, member L
  • DC-SIGNMGC129965
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca2+ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The LWC06 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DC-SIGN/CD209
Antibody (LWC06)
NBP1-43328
DC-SIGN/CD209 Antibody (LWC06)
Species: Mu
Applications: WB, Flow, IP

Publications for DC-SIGN/CD209 Antibody (NBP1-43328)(1)

We have publications tested in 1 confirmed species: Mouse.


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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43328AF350
Alexa Fluor 405 NBP1-43328AF405
Alexa Fluor 488 NBP1-43328AF488
Alexa Fluor 532 NBP1-43328AF532
Alexa Fluor 594 NBP1-43328AF594
Alexa Fluor 647 NBP1-43328AF647
Alexa Fluor 700 NBP1-43328AF700
Alexa Fluor 750 NBP1-43328AF750
Allophycocyanin NBP1-43328APC
Allophycocyanin/Cy7 NBP1-43328APCCY7
Biotin NBP1-43328B
DyLight 350 NBP1-43328UV
DyLight 405 NBP1-43328V
DyLight 488 NBP1-43328G
DyLight 550 NBP1-43328R
DyLight 594 NBP1-43328DL594
DyLight 650 NBP1-43328C
DyLight 680 NBP1-43328FR
DyLight 755 NBP1-43328IR
FITC NBP1-43328F
FITC NBP1-43965
HRP NBP1-43328H
Janelia Fluor 549 NBP1-43328JF549
Janelia Fluor 646 NBP1-43328JF646
PE NBP1-43790
PE NBP1-43328PE
PE NBP1-43790
PE/Atto594 NBP1-43328PEATT594
PE/Cy5.5 NBP1-43328PECY55
PE/Cy7 NBP1-43328PECY7
PerCP NBP1-43328PCP
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Gene Symbol CD209
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