![Western Blot: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Western Blot Analysis of human liver and spleen tissue lysates using DC-SIGN/CD209 Antibody (C209/1781).](http://images.novusbio.com/fullsize2/DC-SIGN-CD209-Antibody-(C209-1781)---Azide-and-BSA-Free-Western-Blot-NBP2-54351-img0004.jpg "Western Blot: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Western Blot Analysis of human liver and spleen tissue lysates using DC-SIGN/CD209 Antibody (C209/1781).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready |
| Clone | C209/1781 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human CD209 protein fragment |
| Localization | Secreted and Cell Surface |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD209 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | IHC-P:Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA Buffer pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes |
| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54351AF350 | |
| Alexa Fluor 405 | NBP2-54351AF405 | |
| Alexa Fluor 488 | NBP2-54351AF488 | |
| Alexa Fluor 532 | NBP2-54351AF532 | |
| Alexa Fluor 594 | NBP2-54351AF594 | |
| Alexa Fluor 647 | NBP2-54351AF647 | |
| Alexa Fluor 700 | NBP2-54351AF700 | |
| Alexa Fluor 750 | NBP2-54351AF750 | |
| Allophycocyanin | NBP2-54351APC | |
| Allophycocyanin/Cy7 | NBP2-54351APCCY7 | |
| Biotin | NBP2-54351B | |
| DyLight 350 | NBP2-54351UV | |
| DyLight 405 | NBP2-54351V | |
| DyLight 488 | NBP2-54351G | |
| DyLight 550 | NBP2-54351R | |
| DyLight 594 | NBP2-54351DL594 | |
| DyLight 650 | NBP2-54351C | |
| DyLight 680 | NBP2-54351FR | |
| DyLight 755 | NBP2-54351IR | |
| FITC | NBP2-54351F | |
| HRP | NBP2-54351H | |
| Janelia Fluor 549 | NBP2-54351JF549 | |
| Janelia Fluor 646 | NBP2-54351JF646 | |
| PE | NBP2-54351PE | |
| PE/Atto594 | NBP2-54351PEATT594 | |
| PE/Cy5.5 | NBP2-54351PECY55 | |
| PE/Cy7 | NBP2-54351PECY7 | |
| PerCP | NBP2-54351PCP |
Diseases for DC-SIGN/CD209 Antibody (NBP2-54351)Discover more about diseases related to DC-SIGN/CD209 Antibody (NBP2-54351).
| Pathways for DC-SIGN/CD209 Antibody (NBP2-54351)View related products by pathway.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CD209 |
![Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).](http://images.novusbio.com/fullsize2/DC-SIGN-CD209-Antibody-(C209-1781)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54351-img0002.jpg "Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).")
![Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).](http://images.novusbio.com/fullsize2/DC-SIGN-CD209-Antibody-(C209-1781)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54351-img0001.jpg "Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).")
![Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Formalin-fixed, paraffin-embedded human Small Intestine stained with CD209 Monoclonal Antibody (C209/1781).](http://images.novusbio.com/fullsize2/DC-SIGN-CD209-Antibody-(C209-1781)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54351-img0003.jpg "Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) - Azide and BSA Free [NBP2-54351] - Formalin-fixed, paraffin-embedded human Small Intestine stained with CD209 Monoclonal Antibody (C209/1781).")
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Western-Blot-NBP1-92684-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM-3.](http://images.novusbio.com/images2/ICAM-3-CD50-Antibody-(OTI1E7)---Azide-and-BSA-Free-Western-Blot-NBP2-70975-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ICAM3.](http://images.novusbio.com/images2/ICAM-3-CD50 Antibody (OTI1E7) - Azide and BSA Free-Immunocytochemistry-Immunofluorescence-NBP2-70975-img0008.jpg)
![Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) - Azide and BSA Free [NBP2-70975] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/ICAM-3-CD50-Antibody-(OTI1E7)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-70975-img0007.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/images2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/images2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Western Blot: NDUFA2 Antibody [NBP2-93743] - Analysis of extracts of various cell lines, using NDUFA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/images2/NDUFA2 Antibody-Western Blot-NBP2-93743-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFA2 Antibody [NBP2-93743] - Analysis of NIH/3T3 cells using NDUFA2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/NDUFA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93743-img0001.jpg)
![Immunohistochemistry-Paraffin: NDUFA2 Antibody [NBP2-93743] - Paraffin-embedded human liver cancer using NDUFA2.](http://images.novusbio.com/images2/NDUFA2-Antibody-Immunohistochemistry-Paraffin-NBP2-93743-img0003.jpg)
![Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Knockout-Validated-NBP2-25208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Western-Blot-NBP2-25255-img0010.jpg)
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Immunohistochemistry-Paraffin-NBP2-25255-img0002.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Flow-Cytometry-NBP2-25255-img0009.jpg)
![Immunohistochemistry: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - IHC on mouse lungcryosection](http://images.novusbio.com/images2/DC-LAMP-Antibody-(1010E1.01)-Immunohistochemistry-DDX0191P-100-img0015.jpg)
![Immunohistochemistry-Paraffin: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - Paraffin embedded lung tumor section stained with DC-LAMP](http://images.novusbio.com/images2/DC-LAMP-Antibody-(1010E1.01)-Immunohistochemistry-Paraffin-DDX0191P-100-img0006.jpg)
![Immunohistochemistry-Paraffin: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - Staining of sentinel lymph nodes from 458 patients with DC-LAMP](http://images.novusbio.com/images2/DC-LAMP-Antibody-(1010E1.01)-Immunohistochemistry-Paraffin-DDX0191P-100-img0007.jpg)
![Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Dual-RNAscope-ISH-IHC-NB100-56566-img0036.jpg)
![Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood. PBMC were stained in a 2 color flow test, with CD14 PE version of this antibody and 1 ug of either isotype control (left) or TLR4-Alexa Fluor 647 (right). PPI negative, CD14+ cells were gated for analysis.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Flow-Cytometry-NB100-56566-img0024.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Immunohistochemistry-NB100-56566-img0040.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (KP1) [NB100-683] - GroEL induces cytokines expression and macrophages as well as osteoclasts infiltration in rat gingival. Representative images (40x) of infiltrated macrophages stained with anti-CD 68 in the gingiva. The black arrowheads indicate the infiltrated macrophages. The right graphs show the quantitative data of CD68-positive cells in HPF (magnification of 200x). Data are expressed as the mean +/- SEM of three slides. *p<0.05 compared with control cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0102450) licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD68-SR-D1-Antibody-(KP1)-Immunohistochemistry-NB100-683-img0008.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68/SR-D1 Antibody NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1:50 for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/images2/CD68-SR-D1-Antibody-(KP1)-Flow-Cytometry-NB100-683-img0004.jpg)
![Western Blot: CD68/SR-D1 Antibody (KP1) [NB100-683] - Total protein from mouse Raw cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD68 (NB100-683) in blocking buffer and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/images2/CD68-SR-D1-Antibody-(KP1)-Western-Blot-NB100-683-img0007.jpg)
