DAX1/NR0B1 Antibody

Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NLS5716] - Anti-NR0B1 / DAX1 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NLS5716] - Analysis of anti-DAX1 antibody with human adrenal.

Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NLS5716] - Anti-NR0B1 / DAX1 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Discontinued

DAX1/NR0B1 Antibody Summary

• Immunogen: Synthetic 20 amino acid peptide from C-terminus of human DAX1.
• Specificity: Human DAX1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Orangutan (100%), Chimpanzee (100%), Primate (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR0B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 11 ug/ml
• Control:

  DAX1/NR0B1 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (95%), Gibbon (90%), Marmoset (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAX1/NR0B1 Antibody

• AHC
• AHCDSS
• AHCH
• AHX
• DAX1
• DAX1DAX-1
• dosage-sensitive sex reversal
• DSS-AHC critical region on the X chromosome protein 1
• GTD
• HHG
• NR0B1
• NROB1
• nuclear hormone receptor
• Nuclear receptor DAX-1
• nuclear receptor subfamily 0 group B member 1
• nuclear receptor subfamily 0, group B, member 1

Background

DAX-1 (DSS-AHC critical region on the X chromosome, gene 1) is an orphan NRO Knirps-Like nuclear receptor that is involved in adrenal gland and gonad development. DAX-1 has been shown to negatively regulate transcription of the nuclear receptor steroidogenic factor 1 (SF-1). DAX-1 is also involved in the development and differentiation of anterior pituitary cells and in steroidogenesis. In the nucleus, DAX-1 binds to a retinoic acid-responsive element (RARE) and acts as a dominant-negative regulator of retinoic acid receptor-mediated transcription. DAX-1 gene transcription is regulated by Ad4BP/SF-1 (NR5A1) and is suppressed by androgens and the androgen receptor (AR/NR3C4); AR down-regulated DAX-1 gene transcription is mediated by Ad4BP/SF-1 in the presence of the ligand. Recently it has been shown that DAX-1 is required for testis development (Meeks et al. 2003). Mutations in the human DAX-1 gene lead to X-linked adrenal hypoplasia congenita and hypogonadotropic hypogonadism. Recently two isoforms of DAX-1 has been identified. DAX-1 expression has been documented in human adrenal, brain, and testis. ESTs have been isolated from cancerous human bladder, germ cell, and lung, as well as normal human brain, pancreas and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR0B1
• Entrez:
  • 190(Human)