DAD1 Antibody - BSA Free

Western Blot: DAD1 Antibody [NB600-669] - Analysis of whole cell lysates from HepG2 human hepatocellular carcinoma cells probed with Rabbit anti Human DAD1

Immunocytochemistry/Immunofluorescence: DAD1 Antibody [NB600-669] - Immunocytochemical stainning of HepG2 cells with Rabbit anti Human DAD1

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DAD1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to a fourteen amino acid sequence within the C-Terminal region of human DAD1
• Localization: Cytoplasmic (microsome)
• Specificity: DAD1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DAD1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: WB: Use at a concentration of 0.5 - 2.0 ug/ml. Detects a band of approximately 22 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Predicted cross-reactivities: Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: IgG purified

Alternate Names for DAD1 Antibody - BSA Free

• DAD-1
• defender against cell death 1EC 2.4.1.119
• dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• Oligosaccharyl transferase subunit DAD1
• oligosaccharyltransferase 2 homolog
• OST2

Background

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAD1 Antibody (NB600-669)(1)

Publication using NB600-669

• Espinoza S, Scarpato M, Damiani D et al. SINEUP non-coding RNA targeting GDNF rescues motor deficits and neurodegeneration in a mouse model of Parkinson s Disease Molecular Therapy 2019-08-01 [PMID: 31495777] (WB, Mouse)

Bioinformatics

• Gene Symbol: DAD1
• Entrez:
  • 1603(Human)
  • 13135(Mouse)
• Uniprot:
  • P61803(Human)