Cytokeratin 19 Antibody

Western Blot: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in MCF7 lysate.

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Antibody at 1:25 in MCF7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Confocal immunofluorescent analysis of HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Simple Western: Cytokeratin 19 Antibody [NBP1-78278] - Image shows a specific band for Cytokeratin 19 in 0.05 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.16 mg/ml

Cytokeratin 19 Antibody Summary

• Immunogen: A synthetic peptide made to an N-terminal portion of the human Cytokeratin 19 protein (between residues 1-50) [UniProt P08727]
• Localization: Cytoplasmic.
• Marker: Epithelial Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT19
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Simple Western 1:3000
  • Immunocytochemistry/Immunofluorescence 1:75
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: In Western blot a band is seen at ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  MCF-7 Whole Cell Lysate
  HepG2 Whole Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27070090).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.16 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytokeratin 19 Antibody

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Background

Cytokeratin 19 (KRT19) is an intermediate filament (IF) protein that is part of the Keratin family of proteins. This protein is also known by the names of Keratin 19 or Keratin type I Cytoskeletal 19. The Keratin family consists of Keratin 1-20, which are divided into two subtypes: Type 1 contains the acidic Cytokeratin proteins consisting of Cytokeratins 9-20, including KRT19; Type 2 Keratins include the basic to neutral protein and contains Cytokeratin 1-8. Type 1 and Type 2 Keratins often form oligomers, interacting to create heterotypic chains. However, KRT19 is unique in that while being the smallest of the Type 1 Keratin proteins, it does not interact with any Type 2 Keratins. IF proteins such as KRT19 are integral for defining and maintaining cellular structure and morphology, and are important for cellular integrity by anchoring to membrane complexes via proteins such as Epiplakin, Trichoplein, Periplakin, Plakophilins, Plectin, BPAG1, Desmoplakin, and Envoplakin. KRT19 is also involved in the organization of the myofibers of striated muscle. KRT19 and other Cytokeratin proteins are mainly found in epithelial cells, with KRT19 being expressed exclusively in defined zones of basal keratinocytes. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 19 Antibody (NBP1-78278)(2)

Publications using NBP1-78278

• Mela M. Marcatori molecolari di cellule staminali nella cancerogenesi epatica sperimentale. Thesis. 2016 (IHC-P, WB, Rat)
• Kowalik MA, Guzzo G, Morandi A et al. Metabolic reprogramming identifies the most aggressive lesions at early phases of hepatic carcinogenesis. Oncotarget. May 31 2016 [PMID: 27070090] (Rat)

Reviews for Cytokeratin 19 Antibody (NBP1-78278) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anony- mous ICC Human 06/09/2014

Summary

• Application: Immunocytochemistry
• Species: Human
• Special Applications: Confocal immunofluorescent analysis of HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
• File: View PDF

reviewed by: Anony- mous WB Human 06/09/2014

Summary

• Application: Western Blot
• Species: Human
• Special Applications: Western blot analysis of extracts from MCF7 and HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:100).
• File: View PDF

FAQs for Cytokeratin 19 Antibody (NBP1-78278). (Showing 1 - 1 of 1 FAQs).

1. Would you be able to confirm whether or not NBP1-78278 - Cytokeratin 19 Antibody is mouse reactive?
   • We have not yet tested our product NBP1-78278 with mouse samples. Based on an alignment of the immunogen used to raise this product and the mouse sequence, this product shares 71% homology with the mouse protein.

Bioinformatics

• Gene Symbol: KRT19
• Entrez:
  • 3880(Human)
• Uniprot:
  • P08727()