Cytokeratin 19 Antibody

Images

 
Western Blot: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in MCF7 lysate.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Antibody at 1:25 in MCF7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Confocal immunofluorescent analysis of HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:5). An Alexa Fluor 488-conjugated Goat to ...read more
Simple Western: Cytokeratin 19 Antibody [NBP1-78278] - Image shows a specific band for Cytokeratin 19 in 0.05 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.16 mg/ml

Order Details

Cytokeratin 19 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human Cytokeratin 19 protein (between residues 1-50) [UniProt P08727]
Localization
Cytoplasmic.
Marker
Epithelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT19
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 1:3000
  • Immunocytochemistry/Immunofluorescence 1:75
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
In Western blot a band is seen at ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
MCF-7 Lysate (NB800-PC5)
HepG2 Lysate (NBP1-42569)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-78278 in the following applications:

Publications
Read Publications using
NBP1-78278 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27070090).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.16 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 19 Antibody

  • CK19
  • CK-19
  • Cytokeratin 19
  • Cytokeratin-19,40-kDa keratin intermediate filament
  • EndoC
  • K19
  • K19cytokeratin-19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Background

Cytokeratin 19 (KRT19) is an intermediate filament (IF) protein that is part of the Keratin family of proteins. This protein is also known by the names of Keratin 19 or Keratin type I Cytoskeletal 19. The Keratin family consists of Keratin 1-20, which are divided into two subtypes: Type 1 contains the acidic Cytokeratin proteins consisting of Cytokeratins 9-20, including KRT19; Type 2 Keratins include the basic to neutral protein and contains Cytokeratin 1-8. Type 1 and Type 2 Keratins often form oligomers, interacting to create heterotypic chains. However, KRT19 is unique in that while being the smallest of the Type 1 Keratin proteins, it does not interact with any Type 2 Keratins. IF proteins such as KRT19 are integral for defining and maintaining cellular structure and morphology, and are important for cellular integrity by anchoring to membrane complexes via proteins such as Epiplakin, Trichoplein, Periplakin, Plakophilins, Plectin, BPAG1, Desmoplakin, and Envoplakin. KRT19 is also involved in the organization of the myofibers of striated muscle. KRT19 and other Cytokeratin proteins are mainly found in epithelial cells, with KRT19 being expressed exclusively in defined zones of basal keratinocytes. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 19 Antibody (NBP1-78278)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Rat
(2)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP1-78278 Applications Species
Mela M. Marcatori molecolari di cellule staminali nella cancerogenesi epatica sperimentale. Thesis. 2016 (IHC-P, WB, Rat) IHC-P, WB Rat
Kowalik MA, Guzzo G, Morandi A et al. Metabolic reprogramming identifies the most aggressive lesions at early phases of hepatic carcinogenesis. Oncotarget. May 31 2016 [PMID: 27070090] (Rat) Rat

Reviews for Cytokeratin 19 Antibody (NBP1-78278) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-78278:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Cytokeratin 19 NBP1-78278
Enlarge
reviewed by:
Anony- mous
ICC Human 06/09/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsConfocal immunofluorescent analysis of HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot Cytokeratin 19 NBP1-78278
Enlarge
reviewed by:
Anony- mous
WB Human 06/09/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from MCF7 and HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:100).
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 19 Antibody (NBP1-78278). (Showing 1 - 1 of 1 FAQs).

  1. Would you be able to confirm whether or not NBP1-78278 - Cytokeratin 19 Antibody is mouse reactive?
    • We have not yet tested our product NBP1-78278 with mouse samples. Based on an alignment of the immunogen used to raise this product and the mouse sequence, this product shares 71% homology with the mouse protein.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-78278B
DyLight 405 Labeled NBP1-78278V
DyLight 488 Labeled NBP1-78278G
DyLight 550 Labeled NBP1-78278R
DyLight 650 Labeled NBP1-78278C
FITC Labeled NBP1-78278F
HRP Labeled NBP1-78278H

Additional Array Products

Bioinformatics Tool for Cytokeratin 19 Antibody (NBP1-78278)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anony- mous
06/09/2014
Application: ICC
Species: Human

Anony- mous
06/09/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol KRT19
Entrez
Uniprot