Cytokeratin 10 Antibody (3C2F5) - BSA Free

Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of esophageal cancer tissues using KRT10 mouse mAb with DAB staining.

Flow Cytometry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).

Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against human KRT10 (AA: 345-454) recombinant protein. (Expected MW is 38.7 kDa)

Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mouse mAb against A431 (1), C6 (2), COS7 (3), Jurkat (4), NIH/3T3 (5), and HEK293 (6) cell lysate.

Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of breast cancer tissues using KRT10 mouse mAb with DAB staining.

ELISA: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, Flow, IHC
• Clone: 3C2F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cytokeratin 10 Antibody (3C2F5) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human KRT10 (AA: 345-454 ) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:100-1:2000
• Theoretical MW: 58.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Cytokeratin 10 Antibody (3C2F5) - BSA Free

• BCIE
• BIE
• CK10
• CK-10
• cytokeratin 10
• Cytokeratin-10
• EHK
• K10keratosis palmaris et plantaris
• Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris)
• keratin 10
• keratin, type I cytoskeletal 10
• keratin-10
• KPP

Background

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 10 Antibody (NBP2-61736)(1)

Publication using NBP2-61736

• Xu h, Wang Z, Yang H et al. Epidermal stem cell-derived exosomes alleviate autophagy induced endothelial cell apoptosis through miR200b-3p/ERK pathway in diabetic wounds Research Square 2022-06-16 (FLOW, Mouse)

Bioinformatics

• Gene Symbol: KRT10