Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free

Western Blot: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT18.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human liver tissue within the normal limits. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, (1:6000).

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Western Blot: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.

Western Blot: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Cytokeratin 18 antibody ( 1E1) at 1:1000 dilution with HepG2 cell lysate

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human colon tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human pancreas tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI1E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free (NBP1-47817) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1000
• Theoretical MW: 47.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3)
• Preservative: 8% Trehalose
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18