Cytokeratin 18 Antibody (OTI1E1)

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT18.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Extracts (10ug) from 10 Human tissue by using Cytokeratin 18 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Cytokeratin 18 antibody ( 1E1) at 1:1000 dilution with HepG2 cell lysate

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human liver tissue within the normal limits. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, (1:6000).

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI1E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cytokeratin 18 Antibody (OTI1E1) Summary

• Description: Novus Biologicals Mouse Cytokeratin 18 Antibody (OTI1E1) (NBP1-47817) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.
• Marker: Epithelial Marker
• Specificity: This antibody is specific for Homo sapiens keratin 18 (KRT18), transcript variant 2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1000
• Theoretical MW: 47.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytokeratin 18 Antibody (OTI1E1)

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

Keratin 18 together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Cytokeratin 18 Antibody (NBP1-47817) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/09/2023

Summary

• Application: Western Blot
• Sample Tested: PANC-1 human pancreatic carcinoma cell line
• Species: Human
• Lot: A002

Bioinformatics

• Gene Symbol: KRT18
• Entrez:
  • 3875(Human)