Cytokeratin 18 Antibody (C-04) [FITC]

• Reactivity: Ma
• Applications: Flow
• Clone: C-04
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC
• Concentration: 0.1 mg/ml

Cytokeratin 18 Antibody (C-04) [FITC] Summary

• Immunogen: Cytoskeleton preparation of epidermal carcinoma cell line A431.
• Marker: Epithelial Marker
• Specificity: The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1-2 ug/ml
  • Flow Cytometry 1-2 ug/ml
• Application Notes: Western blot, IHC and Flow Cytometry. IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Predicted molecular weight: 45 kDa. Optimal dilutions/concentrations should be determined by the end user.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Size Exclusion Chromatography

Alternate Names for Cytokeratin 18 Antibody (C-04) [FITC]

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

Cytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18