Cytokeratin 18 Antibody (C-04) [FITC]

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Product Details

Summary
Reactivity Hu, Mu, MaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
C-04
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC
Concentration
0.1 mg/ml

Cytokeratin 18 Antibody (C-04) [FITC] Summary

Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Marker
Epithelial Marker
Specificity
The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT18
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:50
Application Notes
Western blot, IHC and Flow Cytometry. IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Predicted molecular weight: 45 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
15mM Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 18 Antibody (C-04) [FITC]

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Background

Cytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 18 Antibody (NBP1-48350) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18
Entrez