| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC-Fr, IHC-P |
| Clone | LL002 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Biotin |
| Immunogen | A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14 |
| Localization | Cytoplasmic |
| Marker | Squamous Cell Marker |
| Specificity | Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast. |
| Isotype | IgG3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT14 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Readout System |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for Cytokeratin 14 Antibody (NBP2-34675B)Discover more about diseases related to Cytokeratin 14 Antibody (NBP2-34675B).
| Pathways for Cytokeratin 14 Antibody (NBP2-34675B)View related products by pathway.
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![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(EP1601Y)-Immunocytochemistry-Immunofluorescence-NB110-56916-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(EP1601Y)-Immunohistochemistry-Paraffin-NB110-56916-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 antibody labels squamous elements in the basal epidermis and sebaceous glands.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(EP1601Y)-Immunohistochemistry-Paraffin-NB110-56916-img0010.jpg)
![Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/Cytokeratin-10-Antibody-(3C2F5)-Western-Blot-NBP2-61736-img0006.jpg)
![Immunohistochemistry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of paraffin-embedded esophageal cancer tissues using KRT10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Cytokeratin-10-Antibody-(3C2F5)-Immunohistochemistry-NBP2-61736-img0004.jpg)
![Flow Cytometry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/Cytokeratin-10-Antibody-(3C2F5)-Flow-Cytometry-NBP2-61736-img0002.jpg)
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1. HepG2 and 2. MCF7 lysates.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - MCF7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0004.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0007.jpg)
![Western Blot: CKAP4/p63 Antibody [NBP1-26642] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641and NBP1-26643.](http://images.novusbio.com/fullsize2/CKAP4-p63-Antibody-Western-Blot-NBP1-26642-img0001.jpg)
![Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-26642] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize2/CKAP4-p63-Antibody-Immunohistochemistry-Paraffin-NBP1-26642-img0002.jpg)
![Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Cytokeratin-8-Antibody-Western-Blot-NBP2-16094-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: KRT8 protein stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize2/Cytokeratin 8 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16094-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Cytokeratin 8 Antibody-Immunohistochemistry-Paraffin-NBP2-16094-img0003.jpg)
![Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).](http://images.novusbio.com/fullsize2/RPE65-Antibody-(401.8B11.3D9)-Immunohistochemistry-Frozen-NB100-355-img0007.jpg)
![Western Blot: RPE65 Antibody (401.8B11.3D9) [NB100-355] - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.](http://images.novusbio.com/fullsize2/RPE65-Antibody-(401.8B11.3D9)-Western-Blot-NB100-355-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.](http://images.novusbio.com/fullsize2/RPE65-Antibody-(401.8B11.3D9)-Immunocytochemistry-Immunofluorescence-NB100-355-img0006.jpg)
![Western Blot: UVRAG Antibody [NBP2-24482] - Analysis of UVRAG in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart using this antibody at 0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.](http://images.novusbio.com/fullsize2/UVRAG Antibody-Western Blot-NBP2-24482-img0001.jpg)
![Western Blot: Cytokeratin 7 Antibody [NBP1-88080] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-Western-Blot-NBP1-88080-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining in human placenta and skeletal muscle tissues. Corresponding KRT7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-88080-img0021.jpg)
![Western Blot: Cytokeratin 7 Antibody [NBP1-88080] - Analysis in human cell line SiHa.](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-Western-Blot-NBP1-88080-img0008.jpg)
![Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).](http://images.novusbio.com/fullsize2/pan-Cytokeratin-Antibody-(AE1-+-AE3)-Immunohistochemistry-Paraffin-NBP2-29429-img0008.jpg)
![Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Pan Cytokeratin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 1.7 ug/mL of mouse anti-pan Cytokeratin monoclonal (NBP2-29429, Novus Biologicals) for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize2/pan-Cytokeratin-Antibody-(AE1-+-AE3)-Immunohistochemistry-Paraffin-NBP2-29429-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - MCF-7 cells stained with Biotin conjugated version of pan Cytokeratin antibody and SAv-A488. A) SAv-A488 only at a dilution of 1:500, B) pan CK at a dilution of 1:200, C) pan CK at a dilution of 1:400, D) pan CK at a dilution of 1:800. Image from verified customer review.](http://images.novusbio.com/fullsize2/pan-Cytokeratin-Antibody-(AE1-+-AE3)-Immunocytochemistry-Immunofluorescence-NBP2-29429-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - analysis of HeLa cell culture stained with chicken pAb to vimentin, NB300-223, dilution 1:10,000 in green, and costained with EnCor mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0001.jpg)
![Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates using chicken pAb to Vimentin, NB300-223, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-NB300-223-img0002.jpg)
![Western Blot: Choline kinase alpha Antibody [NBP1-85630] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-CHKA antibody. Corresponding CHKA RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize2/Choline-kinase-alpha-Antibody-Western-Blot-NBP1-85630-img0008.jpg)
![Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/Choline-kinase-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85630-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Kinase beta Antibody [NBP1-85632] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize2/Choline Kinase beta Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85632-img0005.jpg)
![Immunohistochemistry-Paraffin: Choline Kinase beta Antibody [NBP1-85632] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize2/Choline-Kinase-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-85632-img0004.jpg)
![Western Blot: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/Cytokeratin 16 Antibody (SC52-09)-Western Blot-NBP2-67559-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Cytokeratin-16-Antibody-(SC52-09)-Immunocytochemistry-Immunofluorescence-NBP2-67559-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Cytokeratin-16-Antibody-(SC52-09)-Immunohistochemistry-Paraffin-NBP2-67559-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo](http://images.novusbio.com/fullsize2/Cytokeratin 18 Antibody (RGE53)-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0006.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of swine liver hepatocytes](http://images.novusbio.com/fullsize2/Cytokeratin 18 Antibody (RGE53)-Immunohistochemistry-Frozen-NBP1-97715-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize2/Cytokeratin 18 Antibody (RGE53)-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0004.jpg)
![Western Blot: Cytokeratin 13 Antibody [NBP2-38166] - Analysis in human cell lines A-431 and HEK293 using anti-KRT13 antibody. Corresponding KRT13 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize2/Cytokeratin-13-Antibody-Western-Blot-NBP2-38166-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 13 Antibody [NBP2-38166] - Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.](http://images.novusbio.com/fullsize2/Cytokeratin-13-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38166-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP2-38166] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize2/Cytokeratin-13-Antibody-Immunohistochemistry-Paraffin-NBP2-38166-img0016.jpg)
![Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.](http://images.novusbio.com/images2/Streptavidin-Full-length-Protein-Western-Blot-NBP2-29370-img0002.jpg "Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.")
![SDS-Page: Recombinant Human Cytokeratin 14 Protein [NBP1-72467] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/images2/Recombinant Human Cytokeratin 14 Protein-SDS-Page-NBP1-72467-img0001.jpg "SDS-Page: Recombinant Human Cytokeratin 14 Protein [NBP1-72467] - 15% SDS-PAGE (3ug)")
