Home » Cytokeratin 14 » Cytokeratin 14 Antibodies » Cytokeratin 14 Antibody (LL002) [Biotin]

Cytokeratin 14 Antibody (LL002) [Biotin]

 Submit a Review

Images

 
There are currently no images for Cytokeratin 14 Antibody (NBP2-34675B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P
Clone
LL002
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-34675AF405
Alexa Fluor 488
NBP2-34675AF488
Alexa Fluor 647
NBP2-34675AF647
Alexa Fluor 700
NBP2-34675AF700
Allophycocyanin
NBP2-34675APC
DyLight 350
NBP2-34675UV
DyLight 405
NBP2-34675V
DyLight 488
NBP2-34675G
DyLight 550
NBP2-34675R
DyLight 650
NBP2-34675C
DyLight 680
NBP2-34675FR
DyLight 755
NBP2-34675IR
HRP
NBP2-34675H
PE
NBP2-34675PE
Unconjugated
NBP2-34270-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-34270-0.1mg
Azide and BSA Free
NBP2-34675-0.1 mg
Azide and BSA Free
NBP2-34675-0.2 mg
IHC-Prediluted
NBP2-45029
100% Guarantee on every product

Cytokeratin 14 Antibody (LL002) [Biotin] Summary

Immunogen
A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14
Localization
Cytoplasmic
Marker
Squamous Cell Marker
Specificity
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
KRT14
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cytokeratin 14 Antibody (LL002) [Biotin]

  • CK14
  • CK-14
  • Cytokeratin 14
  • cytokeratin-14
  • EBS3
  • EBS4
  • K14
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14
  • keratin, type I cytoskeletal 14
  • keratin-14
  • KRT14
  • NFJ

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Cytokeratin 5
Antibody (2C2)
NBP2-22194
Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Western blot analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.
Species: Hu, Mu, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Cytokeratin 10
Antibody (SPM261)
NBP2-32962
Flow Cytometry: Cytokeratin 10 Antibody (SPM261) [NBP2-32962] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-32962. An intracellular stain was performed on HeLa cells with Cytokeratin 10 antibody (SPM261) NBP2-34752 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 microgram of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Cytokeratin 19
Antibody
NB100-687
Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1. HepG2 and 2. MCF7 lysates.
Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - MCF7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
p63/TP73L Antibody
[Unconjugated]
AF1916
p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(3 Reviews)
Species: Hu
Applications: WB, IHC, ICC
CKAP4/p63 Antibody
NBP1-26642
Western Blot: CKAP4/p63 Antibody [NBP1-26642] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641and NBP1-26643.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-26642] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).
(1 Publication)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
Cytokeratin 8
Antibody
NBP2-16094
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: KRT8 protein stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
RPE65 Antibody
(401.8B11.3D9)
NB100-355
Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).
Western Blot: RPE65 Antibody (401.8B11.3D9) [NB100-355] - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.
Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.
(6 Reviews)
(43 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, ICC
UVRAG Antibody
NBP2-24482
Western Blot: UVRAG Antibody [NBP2-24482] - Analysis of UVRAG in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart using this antibody at 0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB
Cytokeratin 7
Antibody
NBP1-88080
Western Blot: Cytokeratin 7 Antibody [NBP1-88080] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining in human placenta and skeletal muscle tissues. Corresponding KRT7 RNA-seq data are presented for the same tissues.
Western Blot: Cytokeratin 7 Antibody [NBP1-88080] - Analysis in human cell line SiHa.
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
EGFR Antibody
[Unconjugated]
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(6 Publications)
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
pan Cytokeratin
Antibody (AE1 + AE3)
NBP2-29429
Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).
Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Pan Cytokeratin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 1.7 ug/mL of mouse anti-pan Cytokeratin monoclonal (NBP2-29429, Novus Biologicals) for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).
Immunocytochemistry/Immunofluorescence: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - MCF-7 cells stained with Biotin conjugated version of pan Cytokeratin antibody and SAv-A488. A) SAv-A488 only at a dilution of 1:500, B) pan CK at a dilution of 1:200, C) pan CK at a dilution of 1:400, D) pan CK at a dilution of 1:800. Image from verified customer review.
(9 Publications)
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, Flow-IC
Vimentin Antibody
NB300-223
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - View of mixed neuron/glial cultures stained with NB300-223 (green) and rabbit antibody to GFAP antibody NB300-141(red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
Western Blot: Vimentin Antibody [NB300-223] - Western blot of crude extract of human embryonic kidney Hek293 cells stained with NB300-223, showing a single strong clean band at approx. 50kDa.
(1 Review)
(22 Publications)
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Choline kinase
alpha Antibody
NBP1-85630
Western Blot: Choline kinase alpha Antibody [NBP1-85630] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-CHKA antibody. Corresponding CHKA RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Species: Hu
Applications: WB, IHC, IHC-P
Choline Kinase
beta Antibody
NBP1-85632
Immunocytochemistry/Immunofluorescence: Choline Kinase beta Antibody [NBP1-85632] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: Choline Kinase beta Antibody [NBP1-85632] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Cytokeratin 16
Antibody (OTI2H8)
NBP2-45538
Western Blot: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 16.
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC, IHC-P
Cytokeratin 18
Antibody (RGE53)
NBP1-97715
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo
Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of swine liver hepatocytes
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining
Species: Hu, Mu, Rt, Po, Ca, Ch, Ha, Rb, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, CyTOF-ready
Cytokeratin 13
Antibody
NBP2-38166
Western Blot: Cytokeratin 13 Antibody [NBP2-38166] - Analysis in human cell lines A-431 and HEK293 using anti-KRT13 antibody. Corresponding KRT13 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/Immunofluorescence: Cytokeratin 13 Antibody [NBP2-38166] - Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP2-38166] - Staining in human esophagus and skeletal muscle tissues using anti-KRT13 antibody. Corresponding KRT13 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ErbB2/Her2
[Unconjugated]
1129-ER
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER) blocks anti-ErbB2 mediated inhibition of the SK‑BR‑3 human breast cancer cell line. The ED50 is 20-80 ng/mL in the presence of 0.6 μg/mL Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1129)
(5 Reviews)
(24 Publications)
Species: Hu

Publications for Cytokeratin 14 Antibody (NBP2-34675B) (0)

There are no publications for Cytokeratin 14 Antibody (NBP2-34675B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Cytokeratin 14 Antibody (NBP2-34675B) (0)

There are no reviews for Cytokeratin 14 Antibody (NBP2-34675B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 14 Antibody (NBP2-34675B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cytokeratin 14 Products

Bioinformatics Tool for Cytokeratin 14 Antibody (NBP2-34675B)

Discover related pathways, diseases and genes to Cytokeratin 14 Antibody (NBP2-34675B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytokeratin 14 Antibody (NBP2-34675B)

Discover more about diseases related to Cytokeratin 14 Antibody (NBP2-34675B).
 

Pathways for Cytokeratin 14 Antibody (NBP2-34675B)

View related products by pathway.

PTMs for Cytokeratin 14 Antibody (NBP2-34675B)

Learn more about PTMs related to Cytokeratin 14 Antibody (NBP2-34675B).
 

Research Areas for Cytokeratin 14 Antibody (NBP2-34675B)

Find related products by research area.

Blogs on Cytokeratin 14

There are no specific blogs for Cytokeratin 14, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.
Streptavidin Native Protein
NBP2-29370
Mouse IgG3 Isotype Control (MG3) [Biotin]
NBP1-96891
SDS-Page: Recombinant Human Cytokeratin 14 Protein [NBP1-72467] - 15% SDS-PAGE (3ug)
Recombinant Human Cytokeratin 14 Protein
NBP1-72467-100ug
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
ErbB2/Her2 [Unconjugated]
1129-ER-050

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytokeratin 14 Antibody (LL002) [Biotin] and receive a gift card or discount.


Bioinformatics

Gene Symbol KRT14
Entrez