| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45029 | |||
| Azide and BSA Free | NBP2-34675-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45030 | |||
| Azide and BSA Free | NBP2-34403-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3164-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26869-25ul | |||
| Azide and BSA Free | NBP3-44264 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26868-25ul | |||
| Azide and BSA Free | NBP3-44263 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08531 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89198-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47720-0.1mg | |||
| IHC-Prediluted | NBP2-48042 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84917-25ul | |||
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Immunocytochemistry-Immunofluorescence-NBP2-34270-img0010.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Immunohistochemistry-Paraffin-NBP2-34270-img0011.jpg)
![Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Flow-Cytometry-NBP2-34270-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002)](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Immunohistochemistry-Paraffin-NBP2-34270-img0006.jpg)
![Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Simple Western lane view shows a specific band for Cytokeratin 14 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Simple-Western-NBP2-34270-img0004.jpg)
![Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 14 antibody was used at 10 ug/ml dilution of A431 lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Simple-Western-NBP2-34270-img0005.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0008.jpg "Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0010.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 Antibody (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunocytochemistry-Immunofluorescence-NBP2-34355-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 Antibody (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (20X) stained with Cytokeratin 14 MAb (SPM263).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunohistochemistry-Paraffin-NBP2-34355-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (10X) stained with Cytokeratin 14 MAb (SPM263).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunohistochemistry-Paraffin-NBP2-34355-img0001.jpg)
![Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Enrichment of Cytokeratin 14 (high cells after UTX knockdown in HBLAK cells. Cytokeratin 14 expression as detected by flow cytometry on day 4 after transfection of control siRNA or UTX-siRNA (n = 4). Statistics were performed with a two-way ANOVA with a post-hoc Tukey HSD test (## p < 0.01; ### p < 0.001). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/12/4/1023/htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-34355_mouse-monoclonal-cytokeratin-14-antibody-spm263-1772024936269.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) - Human Tonsil tissue with epithelial CK14 expression as monochromatic image. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-34355_mouse-monoclonal-cytokeratin-14-antibody-spm263-17720241003233.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31326-img0009.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326] - Lung CA, using Cytokeratin 14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-31326-img0004.jpg)
![Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000.](http://images.novusbio.com/fullsize/nbp1-31326_rabbit-polyclonal-cytokeratin-14-antibody-91020242013123.jpg)
![Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31326_rabbit-polyclonal-cytokeratin-14-antibody-9102024195726.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Immunohistochemistry-Paraffin-NBP3-07600-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).")
![Flow Cytometry: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 14 Mouse Monoclonal Antibody ((KRT14/2375) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Flow-Cytometry-NBP3-07600-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Immunohistochemistry-Paraffin-NBP3-07600-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Immunohistochemistry-Paraffin-NBP3-07600-img0003.jpg)
![Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Western-Blot-NBP2-89198-img0003.jpg "Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Immunochemical staining of human Cytokeratin14 in human esophageal carcinoma with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89198-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Staining of human CK14 in human skin with rabbit monoclonal antibody (1:20000).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89198-img0001.jpg)
![Immunoprecipitation: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-KRT14 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody:Anti-KRT14 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size: 52 kDa](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunoprecipitation-NBP2-89198-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/532) [NBP2-45032] - Human Tonsil stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-532-Immunohistochemistry-Paraffin-NBP2-45032-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/532) [NBP2-45032] - Human Tonsil stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/532) [NBP2-45032] - Human Cervix stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-532-Immunohistochemistry-Paraffin-NBP2-45032-img0001.jpg)
![Western Blot: Cytokeratin 14 Antibody [NBP1-84917] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Western-Blot-NBP1-84917-img0005.jpg "Western Blot: Cytokeratin 14 Antibody [NBP1-84917] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-84917] - Staining in human skin and duodenum tissues using anti-KRT14 antibody. Corresponding KRT14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-84917-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-84917] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-84917-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-84917] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-84917-img0010.jpg)
![Western Blot: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Western blot analysis of Cytokeratin 14 on different lysates with Mouse anti-Cytokeratin 14 antibody (NBP3-32242) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (negative) (20 ug/Lane) Lane 3: PANC-1 cell lysate (negative) (20 ug/Lane) Lane 4: Mouse skin tissue lysate (40 ug/Lane) Lane 5: Rat skin tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32242) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32242_mouse-cytokeratin-14-mab-a2c11-r-278202415295571.jpg "Western Blot: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Western blot analysis of Cytokeratin 14 on different lysates with Mouse anti-Cytokeratin 14 antibody (NBP3-32242) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (negative) (20 ug/Lane) Lane 3: PANC-1 cell lysate (negative) (20 ug/Lane) Lane 4: Mouse skin tissue lysate (40 ug/Lane) Lane 5: Rat skin tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32242) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Cytokeratin 14 antibody (NBP3-32242) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32242) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32242_mouse-cytokeratin-14-mab-a2c11-r-29820241435249.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Immunocytochemistry analysis of A431 (positive) and PANC-1 (negative) labeling Cytokeratin 14 with Rabbit anti-Cytokeratin 14 antibody (NBP3-32242) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 14 antibody (NBP3-32242) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32242_mouse-cytokeratin-14-mab-a2c11-r-29202410484820.jpg)