Cytokeratin 16 Antibody (SC52-09)

Western Blot: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 16 with Rabbit anti-Cytokeratin 16 antibody washed with PBS, and then probed with the primary antibody (red) at 1/200 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Flow Cytometry: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16 (washed with PBS. And then probed with the primary antibodies Cytokeratin 16 (red) at 1/200 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SC52-09
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cytokeratin 16 Antibody (SC52-09) Summary

• Description: Novus Biologicals Rabbit Cytokeratin 16 Antibody (SC52-09) (NBP2-67559) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within huamn Cytokeratin 16 aa 27-75 / 473. (SwissProt: P08779 Human)
• Localization: Cytoskeleton, intermediate filament, cytosol, extracellular exosome, nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT16
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 16 Antibody (SC52-09)

• CK16
• CK-16
• cytokeratin 16
• cytokeratin-16
• FNEPPK
• focal non-epidermolytic palmoplantar keratoderma
• K16
• K1CP
• keratin 16
• keratin, type I cytoskeletal 16
• keratin-16
• KRT16A
• NEPPK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT16