Cytokeratin 13 Antibody

Images

 
Western Blot: Cytokeratin 13 Antibody [NBP2-16086] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 13 Antibody [NBP2-16086] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP2-16086] - Immunohistochemical analysis of paraffin-embedded CAL27 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.91 mg/ml

Order Details

Cytokeratin 13 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Cytokeratin 13. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.91 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 13 Antibody

  • CK13
  • CK-13
  • cytokeratin 13
  • Cytokeratin-13
  • K13cytokeratin-13
  • keratin 13
  • keratin, type I cytoskeletal 13
  • keratin-13
  • MGC161462
  • MGC3781

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT13
Uniprot