Recombinant Human SECTM1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SECTM1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5 x 10⁴ cells per well are added to Recombinant Human SECTM1 Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀ for this effect is 1.5-7.5 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human SECTM1 protein
  

  Human SECTM1 (Gln29-Gly145) Accession # Q8WVN6	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q8WVN6
• N-terminal Sequence: Gln29 predicted: No results obtained, sequencing might be blocked
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SECTM1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SECTM1 Fc Chimera Protein, CF

• CD7 Ligand
• K12secreted and transmembrane protein 1
• Protein K12
• Protein K-12
• secreted and transmembrane 1
• SECTM1
• type 1a transmembrane protein

Background

SECTM1 (secreted and transmembrane 1), also called K12, is either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form (1, 2). SECTM1 protein expression is detected in some myeloid cells, such as stimulated monocytes, immature monocyte‑derived dendritic cells, and granulocytes (1‑3). In the thymus, it is also expressed by epithelia and fibroblasts (2). Stimulation with IFN‑ gamma is often necessary to detect SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Human SECTM1 cDNA encodes 248 amino acids (aa), including a 28 aa signal sequence, a 117 aa extracellular domain (ECD) with one potential N‑linked glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic sequence (1). The ECD shows some similarity to Ig domains (1). The human SECTM1 ECD shares limited (approximately 45‑51%) aa sequence identity with mouse SECTM1 (a or b) and rat SECTM1 ECD. SECTM1 from human and mouse each show species‑specific binding of CD7 and co‑stimulate CD4⁺ and CD8⁺ T cells through CD7, either alone or through synergy with CD28 (2‑4). Immobilized recombinant human SECTM1/Fc also enhances human NK cell expression of activation markers such as CD25, CD69 and CD54 (4).

1. Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.
2. Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.
3. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
4. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
5. Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.

Bioinformatics

• Gene Symbol: SECTM1
• Uniprot:
  • Q8WVN6(Human)