Cytokeratin 17 Antibody (E3 (same as Ks17.E3))

Images

 
Western Blot: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Analysis of Cytokeratin 17 in human HeLa lysate using Cytokeratin 17 E3 antibody at 3 ug/ml. goat anti-mouse Ig HRP secondary antibody and ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Formalin-paraffin skin stained with CK17 Ab (E3).
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Formalin-paraffin skin stained with CK17 Ab (E3).
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with CK17 Monoclonal Antibody (E3).
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Formalin-fixed paraffin-embedded human Bladder Carcinoma stained with CK17 Monoclonal Antibody (E3)

Product Details

Summary
Reactivity Hu, Rt, Po, Bv, GtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
E3 (same as Ks17.E3)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) Summary

Immunogen
The cytoskeletal fraction of rat colon epithelium was used as immunogen for the Cytokeratin 17 E3 antibody.
Localization
Cytoplasmic
Marker
Basal Epithelial Marker
Specificity
Cytokeratin 17 (CK17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IFP's). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. CK17 is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5&6, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. CK17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT17
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
Application Notes
Human Chromosome Location: 17q21.2
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Flow Cytometry, Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Frozen have been reported to work in literature. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-29421 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for Cytokeratin 17 Antibody (E3 (same as Ks17.E3))

  • 39.1
  • CK-17
  • cytokeratin-17
  • K17
  • keratin 17
  • keratin, type I cytoskeletal 17
  • keratin-17
  • PC
  • PC2
  • PCHC1

Background

Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant IF protein complement in an epithelial cell-specific manner. The protein encoded by this gene is a type I cytokeratin. It is expressed in abundance in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. It maps to 17q12-q21 region of human chromosome. It may be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 17 Antibody (NBP2-29421)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(1)
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Filter By Species
Human
(2)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 17 Antibody (NBP2-29421) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33188AF350
Alexa Fluor 405 NBP2-33188AF405
Alexa Fluor 488 NBP2-33188AF488
Alexa Fluor 532 NBP2-33188AF532
Alexa Fluor 594 NBP2-33188AF594
Alexa Fluor 647 NBP2-33188AF647
Alexa Fluor 700 NBP2-33188AF700
Alexa Fluor 750 NBP2-33188AF750
Biotin NBP2-33188B
DyLight 350 NBP2-33188UV
DyLight 405 NBP2-33188V
DyLight 488 NBP2-33188G
DyLight 550 NBP2-33188R
DyLight 594 NBP2-33188DL594
DyLight 650 NBP2-33188C
DyLight 680 NBP2-33188FR
DyLight 755 NBP2-33188IR
FITC NBP2-33188F
HRP NBP2-33188H
Janelia Fluor 549 NBP2-33188JF549
Janelia Fluor 646 NBP2-33188JF646

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Bioinformatics

Gene Symbol KRT17
Uniprot