Cytochrome P450 17A1 Antibody

Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842] - (0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NB100-2842] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca, Fe
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cytochrome P450 17A1 Antibody Summary

• Description: Novus Biologicals Goat Cytochrome P450 17A1 Antibody (NB100-2842) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Cytochrome P450 17A1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LFKDGDQKLEK corresponding to internal region according to NP_000093.1.
• Predicted Species: Canine (100%), Feline (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CYP17A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 57.4 kDa band according to NP_000093.1). IHC-P: Human adenal gland shows strong granular staining of the cytoplasm in medulla cells.
• Control:

  Human Ovary Whole Tissue Lysate (Adult Whole Normal)
  Cytochrome P450 17A1 Overexpression Lysate

• Control Peptide:

  Cytochrome P450 17A1 Peptide (NB100-2842PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 17A1 Antibody

• CPT7
• CYP17
• CYP17A1
• CYPXVII
• Cytochrome P450 17A1
• cytochrome p450 XVIIA1
• cytochrome P450, family 17, subfamily A, polypeptide 1
• cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenalhyperplasia
• Cytochrome P450c17
• Cytochrome P450-C17
• EC 1.14.14.19
• EC 1.14.14.32
• EC 1.14.99
• P450C17
• P450C17CYPXVII
• S17AH

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 17A1 Antibody (NB100-2842)(4)

Publications using NB100-2842

• Wang Y, Guo B, Guo Y, Qi N et Al. A spatiotemporal steroidogenic regulatory network in human fetal adrenal glands and gonads Front Endocrinol (Lausanne) 2022-12-05 [PMID: 36465633]
• Efstathiou E, Titus M, Wen S et al. Molecular Characterization of Enzalutamide-treated Bone Metastatic Castration-resistant Prostate Cancer. Eur. Urol. 2014-05-29 [PMID: 24882673] (IHC-P, Human)
• Efstathiou E, Titus M, Tsavachidou D et al. Effects of abiraterone acetate on androgen signaling in castrate-resistant prostate cancer in bone. J Clin Oncol 2012-02-01 [PMID: 22184395] (IHC-P, Human)
• Ohnishi T, Ogawa Y, Saibara T et al. CYP17 polymorphism as a risk factor of tamoxifen-induced hepatic steatosis in breast cancer patients. Oncol Rep 2005-03-01 [PMID: 15706422]

Bioinformatics

• Gene Symbol: CYP17A1
• Entrez:
  • 1586(Human)
• Uniprot:
  • P05093(Human)