Cytochrome P450 17A1 Antibody

Images

 
Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842] - (0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NB100-2842] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Cytochrome P450 17A1 Antibody Summary

Immunogen
Peptide with sequence C-LFKDGDQKLEK corresponding to internal region according to NP_000093.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CYP17A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 57.4 kDa band according to NP_000093.1). IHC-P: Human adenal gland shows strong granular staining of the cytoplasm in medulla cells.
Positive Control
Ovary Lysate (NB820-59243)
Cytochrome P450 17A1 Lysate (NBL1-09673)
Publications
Read Publications using
NB100-2842 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Feline, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytochrome P450 17A1 Antibody

  • CPT7
  • cyp17
  • CYP17A1
  • cytochrome p450 XVIIA1
  • cytochrome P450, family 17, subfamily A, polypeptide 1
  • cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenalhyperplasia
  • Cytochrome P450c17
  • Cytochrome P450-C17
  • EC 1.14.99
  • P450C17
  • P450C17CYPXVII
  • S17AH

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome P450 17A1 Antibody (NB100-2842)(3)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYP17A1
Entrez
Uniprot