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Cytochrome b245 alpha Antibody (CS9)

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Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with Leucoperm

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IP
Clone
CS9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-40974AF350
Alexa Fluor 405
NBP1-40974AF405
Alexa Fluor 488
NBP1-40974AF488
Alexa Fluor 532
NBP1-40974AF532
Alexa Fluor 594
NBP1-40974AF594
Alexa Fluor 647
NBP1-40974AF647
Alexa Fluor 700
NBP1-40974AF700
Alexa Fluor 750
NBP1-40974AF750
Allophycocyanin
NBP1-40974APC
Allophycocyanin/Cy7
NBP1-40974APCCY7
DyLight 350
NBP1-40974UV
DyLight 405
NBP1-40974V
DyLight 488
NBP1-40974G
DyLight 550
NBP1-40974R
DyLight 594
NBP1-40974DL594
DyLight 650
NBP1-40974C
DyLight 680
NBP1-40974FR
DyLight 755
NBP1-40974IR
Janelia Fluor 549
NBP1-40974JF549
Janelia Fluor 646
NBP1-40974JF646
PE
NBP1-40974PE
PE/Atto594
NBP1-40974PEATT594
PE/Cy5.5
NBP1-40974PECY55
PE/Cy7
NBP1-40974PECY7
PerCP
NBP1-40974PCP
100% Guarantee on every product

Cytochrome b245 alpha Antibody (CS9) Summary

Immunogen
Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
Specificity
This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Membrane permeabilisation is required for Flow Cytometry.
Publications
Read Publication using NBP1-40974.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cytochrome b245 alpha Antibody (CS9)

  • cytochrome b light chain
  • Cytochrome b(558) alpha chain
  • cytochrome b(558) alpha-subunit
  • cytochrome b, alpha polypeptide
  • cytochrome b-245 light chain
  • cytochrome b-245, alpha polypeptide
  • Cytochrome b558 subunit alpha
  • flavocytochrome b-558 alpha polypeptide
  • Neutrophil cytochrome b 22 kDa polypeptide
  • p22 phagocyte B-cytochrome
  • p22phox
  • p22-PHOX
  • Superoxide-generating NADPH oxidase light chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.
(2 Reviews)
(2 Publications)
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
NCF1 Antibody
NB100-790
Western Blot: NCF1 Antibody [NB100-790] - Staining of U937 (A), Daudi (B), U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.2, 0.01, and 0.3 ug/mL respectively. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NCF1 Antibody [NB100-790] - Staining of paraffin embedded Human Colon. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(3 Publications)
Species: Hu, Mu, Po, Ba
Applications: WB, Flow, IHC, IHC-P, IP, PEP-ELISA, IF
p67phox/NOXA2
Antibody
NBP1-82542
Western Blot: p67phox/NOXA2 Antibody [NBP1-82542] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunocytochemistry/Immunofluorescence: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human cell line U-251 MG shows localization to nucleoli and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
DCTN3 Antibody
NBP2-98673
Western Blot: DCTN3 Antibody [NBP2-98673] - Anti-DCTN3 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa
Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP2-98673] - Immunochemical staining of human DCTN3 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: DCTN3 Antibody [NBP2-98673] - DCTN3 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-DCTN3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DCTN3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa
Species: Hu
Applications: WB, IHC, IHC-P, IP

Calcium-binding-protei
NBP1-33736
Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE
Immunocytochemistry/Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Calcium-binding-protein-P22 Antibody [NBP1-33736] - Paraffin-embedded mouse lung. Calcium binding protein P22 antibody dilution: 1:500.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
DYNC1H1 Antibody
NBP1-84254
Immunohistochemistry-Paraffin: DYNC1H1 Antibody [NBP1-84254] - Staining in human cerebral cortex and pancreas tissues using anti-DYNC1H1 antibody. Corresponding DYNC1H1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DYNC1H1 Antibody [NBP1-84254] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: DYNC1H1 Antibody [NBP1-84254] - Staining of human pancreas shows low expression as expected.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Prohibitin 2
Antibody
NBP2-13754
Western Blot: Prohibitin 2 Antibody [NBP2-13754] - Analysis in human cell line BEWO.
Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody [NBP2-13754] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Species: Hu, Mu, Rt
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eNOS Antibody
NB300-500
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120328), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
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(17 Publications)
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr, Sh
Applications: WB, IHC, IHC-Fr, IHC-P
Nox4 Antibody
NB110-58849
Immunohistochemistry: Nox4 Antibody [NB110-58849] - AT1-KO mice are resistant to alcohol-induced oxidative stress. Oxidative stress was examined by immunohistochemical staining of NOX4 (D) expression was examined by immunohistochemical staining. Data are presented as means +/- SD. *, P < 0.05 vs corresponding control; #, P < 0.05 vs WT alcohol group. Bar = 50 uM. Image collected and cropped by CiteAb from the following publication (http://doi.wiley.com/10.1111/j.1582-4934.2012.01569.x), licensed under a CC-BY licence.
Western Blot: Nox4 Antibody [NB110-58849] - Detection of NOX4 on IMR90 lysate. Image courtesy of Naomi Logsdon, University of Alabama at Birmingham.
Knockdown Validated: Nox4 Antibody [NB110-58849] - Silencing of NOX4 in H4-II-C3 rat hepatoma cell line. Image from verified customer review.
(8 Reviews)
(84 Publications)
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb, Sh
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P, KD
Angiotensin II/III
Antibody (Ang II E7 ( ...
NB100-62346
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. Western blot analysis for protein expressions of intrarenal RAS components in the two groups of mice. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.
Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical analysis of intrarenal RAS expression position in the two groups of mice. AGT immunoreactivity in the proximal tubular cells, Ang II immunoreactivity in both glomerular and tubular cells, renin immunoreactivity in juxtaglomerular apparatus cells, ACE immunoreactivity in brush border membranes of proximal tubules as well as AT1 and AT2 immunoreactivity in the proximal tubules were increased in the HF group when compared to the control group. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).
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Immunocytochemistry/Immunofluorescence: NOX1 Antibody [NBP1-31546] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-NOX1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Human lung carcinoma. NOX1 antibody diluted at 1:500.
(1 Review)
(22 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(23 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Angiogenin [HRP]
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(20 Publications)
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NCF4 Antibody
NB100-61666
Western Blot: NCF4 Antibody [NB100-61666] - HEK293 overexpressing P40PHOX and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666] - Staining of paraffin embedded Human Spleen at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NCF4 Antibody [NB100-61666] - Analysis of NCF4 antibody using NB100-61666 at 0.01ug/ml in Daudi cell lysate (35ug protein in RIPA buffer).
(1 Publication)
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Applications: WB, IHC, IHC-P, PEP-ELISA
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(35 Reviews)
(649 Publications)
Species: Hu
Rac1 Antibody
NB100-91266
Western Blot: Rac1 Antibody [NB100-91266] - WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution: 1:1000 incubated overnight at 4C.
Immunocytochemistry/Immunofluorescence: Rac1 Antibody [NB100-91266] - Mouse Brain (cerebral cortex). ICC/IF image submitted by a verified customer review.
Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat spinal cord using Rabbit antibody to RAC1, RAC2 (100-150) at 1:1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Nanos3 Antibody
NBP1-76919
Western Blot: Nanos3 Antibody [NBP1-76919] - Human brain tissue lysate with Nanos3 antibody at 2 ug/mL.
Immunocytochemistry/Immunofluorescence: Nanos3 Antibody [NBP1-76919] - in Human Brain cells.
Immunohistochemistry-Paraffin: Nanos3 Antibody [NBP1-76919] - Human brain tissue with Nanos3 antibody at 2.5 ug/ml.
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
SCGF/CLEC11a
Antibody (239029) [Un ...
MAB1904
SCGF/CLEC11a was detected in immersion fixed IFN gamma activated THP-1 human acute monocytic leukemia cell line using 10 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu
Applications: WB, IHC, ICC
AKAP13 Antibody
NBP1-89163
Immunocytochemistry/Immunofluorescence: AKAP13 Antibody [NBP1-89163] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human lymphoid tissues shows strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Cytochrome b245
alpha Antibody (CS9)
NBP1-40974
Cytochrome b245 alpha Antibody (CS9)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IP

Publications for Cytochrome b245 alpha Antibody (NBP1-40974)(1)

We have publications tested in 1 confirmed species: Human.


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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-40974AF350
Alexa Fluor 405 NBP1-40974AF405
Alexa Fluor 488 NBP1-40974AF488
Alexa Fluor 532 NBP1-40974AF532
Alexa Fluor 594 NBP1-40974AF594
Alexa Fluor 647 NBP1-40974AF647
Alexa Fluor 700 NBP1-40974AF700
Alexa Fluor 750 NBP1-40974AF750
Allophycocyanin NBP1-40974APC
Allophycocyanin/Cy7 NBP1-40974APCCY7
DyLight 350 NBP1-40974UV
DyLight 405 NBP1-40974V
DyLight 488 NBP1-40974G
DyLight 550 NBP1-40974R
DyLight 594 NBP1-40974DL594
DyLight 650 NBP1-40974C
DyLight 680 NBP1-40974FR
DyLight 755 NBP1-40974IR
Janelia Fluor 549 NBP1-40974JF549
Janelia Fluor 646 NBP1-40974JF646
PE NBP1-40974PE
PE/Atto594 NBP1-40974PEATT594
PE/Cy5.5 NBP1-40974PECY55
PE/Cy7 NBP1-40974PECY7
PerCP NBP1-40974PCP
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Bioinformatics

Gene Symbol CYBA