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Cytochrome b245 alpha Antibody (CS9) - BSA Free

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Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with Leucoperm

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IP
Clone
CS9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-40974AF350
Alexa Fluor 405
NBP1-40974AF405
Alexa Fluor 488
NBP1-40974AF488
Alexa Fluor 532
NBP1-40974AF532
Alexa Fluor 594
NBP1-40974AF594
Alexa Fluor 647
NBP1-40974AF647
Alexa Fluor 700
NBP1-40974AF700
Alexa Fluor 750
NBP1-40974AF750
Allophycocyanin
NBP1-40974APC
Allophycocyanin/Cy7
NBP1-40974APCCY7
Biotin
NBP1-40974B
DyLight 350
NBP1-40974UV
DyLight 405
NBP1-40974V
DyLight 488
NBP1-40974G
DyLight 550
NBP1-40974R
DyLight 594
NBP1-40974DL594
DyLight 650
NBP1-40974C
DyLight 680
NBP1-40974FR
DyLight 755
NBP1-40974IR
FITC
NBP1-40974F
HRP
NBP1-40974H
Janelia Fluor 549
NBP1-40974JF549
Janelia Fluor 646
NBP1-40974JF646
PE
NBP1-40974PE
PE/Atto594
NBP1-40974PEATT594
PE/Cy5.5
NBP1-40974PECY55
PE/Cy7
NBP1-40974PECY7
PerCP
NBP1-40974PCP
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-40974-0.025mg
100% Guarantee on every product

Cytochrome b245 alpha Antibody (CS9) - BSA Free Summary

Immunogen
Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
Specificity
This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Membrane permeabilisation is required for Flow Cytometry.
Publications
Read Publication using NBP1-40974.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cytochrome b245 alpha Antibody (CS9) - BSA Free

  • cytochrome b light chain
  • Cytochrome b(558) alpha chain
  • cytochrome b(558) alpha-subunit
  • cytochrome b, alpha polypeptide
  • cytochrome b-245 light chain
  • cytochrome b-245, alpha polypeptide
  • Cytochrome b558 subunit alpha
  • flavocytochrome b-558 alpha polypeptide
  • Neutrophil cytochrome b 22 kDa polypeptide
  • p22 phagocyte B-cytochrome
  • p22phox
  • p22-PHOX
  • Superoxide-generating NADPH oxidase light chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm
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(3 Publications)
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Western Blot: NCF1 Antibody [NB100-790] - Staining of U937 (A), Daudi (B), U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.2, 0.01, and 0.3 ug/mL respectively. Detected by chemiluminescence.
Immunofluorescence: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: NCF1 Antibody [NB100-790] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/Immunofluorescence: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human cell line U-251 MG shows localization to nucleoli and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Analysis in human bone marrow and skeletal muscle tissues. Corresponding p67phox/NOXA2 RNA-seq data are presented for the same tissues.
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Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Western Blot: DCTN3 Antibody [NBP1-52105] - Staining (0.2ug/ml) of Mouse (A) and Rat (B) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Immunocytochemistry/Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
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NBP1-84254
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NBP2-13754
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Western Blot: Nanos3 Antibody [NBP1-76919] - Human brain tissue lysate with Nanos3 antibody at 2 ug/mL.
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Species: Hu, Mu, Rt
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Antibody (239029) [Un ...
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Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: AKAP13 Antibody [NBP1-89163] - Staining of human heart shows moderate to strong cytoplasmic positivity in cardiomyocytes.
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Cytochrome b245
alpha Antibody (CS9) ...
NBP1-40974
Cytochrome b245 alpha Antibody (CS9) - BSA Free
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IP

Publications for Cytochrome b245 alpha Antibody (NBP1-40974)(1)

We have publications tested in 1 confirmed species: Human.


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Bioinformatics

Gene Symbol CYBA