Cytochrome b245 alpha Antibody (CS9)

Images

 
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.
Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with Leucoperm

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IP
Clone
CS9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Cytochrome b245 alpha Antibody (CS9) Summary

Immunogen
Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
Specificity
This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Membrane permeabilisation is required for Flow Cytometry.
Publications
Read Publication using NBP1-40974.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cytochrome b245 alpha Antibody (CS9)

  • cytochrome b light chain
  • Cytochrome b(558) alpha chain
  • cytochrome b(558) alpha-subunit
  • cytochrome b, alpha polypeptide
  • cytochrome b-245 light chain
  • cytochrome b-245, alpha polypeptide
  • Cytochrome b558 subunit alpha
  • flavocytochrome b-558 alpha polypeptide
  • Neutrophil cytochrome b 22 kDa polypeptide
  • p22 phagocyte B-cytochrome
  • p22phox
  • p22-PHOX
  • Superoxide-generating NADPH oxidase light chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome b245 alpha Antibody (NBP1-40974)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-40974AF350
Alexa Fluor 405 NBP1-40974AF405
Alexa Fluor 488 NBP1-40974AF488
Alexa Fluor 532 NBP1-40974AF532
Alexa Fluor 594 NBP1-40974AF594
Alexa Fluor 647 NBP1-40974AF647
Alexa Fluor 700 NBP1-40974AF700
Alexa Fluor 750 NBP1-40974AF750
Allophycocyanin NBP1-40974APC
Allophycocyanin/Cy7 NBP1-40974APCCY7
DyLight 350 NBP1-40974UV
DyLight 405 NBP1-40974V
DyLight 488 NBP1-40974G
DyLight 550 NBP1-40974R
DyLight 594 NBP1-40974DL594
DyLight 650 NBP1-40974C
DyLight 680 NBP1-40974FR
DyLight 755 NBP1-40974IR
Janelia Fluor 549 NBP1-40974JF549
Janelia Fluor 646 NBP1-40974JF646
PE NBP1-40974PE
PE/Atto594 NBP1-40974PEATT594
PE/Cy5.5 NBP1-40974PECY55
PE/Cy7 NBP1-40974PECY7
PerCP NBP1-40974PCP

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Bioinformatics

Gene Symbol CYBA
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