Cytochrome b245 alpha Antibody (CS9) [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IP
• Clone: CS9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

Cytochrome b245 alpha Antibody (CS9) [Alexa Fluor™ Plus 680] Summary

• Immunogen: Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
• Specificity: This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYBA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cytochrome b245 alpha Antibody (CS9) [Alexa Fluor™ Plus 680]

• cytochrome b light chain
• Cytochrome b(558) alpha chain
• cytochrome b(558) alpha-subunit
• cytochrome b, alpha polypeptide
• cytochrome b-245 light chain
• cytochrome b-245, alpha polypeptide
• Cytochrome b558 subunit alpha
• flavocytochrome b-558 alpha polypeptide
• Neutrophil cytochrome b 22 kDa polypeptide
• p22 phagocyte B-cytochrome
• p22phox
• p22-PHOX
• Superoxide-generating NADPH oxidase light chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYBA