CYP27B1 Overexpression Lysate (Native)

Images

 
Western Blot: CYP27B1 Overexpression Lysate (Adult Normal) [NBL1-09682] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP27B1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CYP27B1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC222840 and based on accession number NM_000785. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein, mRNA.
Gene
CYP27B1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
56.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CYP27B1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CYP27B1 Overexpression Lysate (Native)

  • 1alpha(OH)ase
  • 25 hydroxyvitamin D3-1-alpha hydroxylase
  • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
  • 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
  • 25-OHD-1 alpha-hydroxylase
  • Calcidiol 1-monooxygenase
  • CP2B
  • CYP1
  • CYP1alpha
  • CYP27B
  • Cytochrome p450 27B1
  • Cytochrome P450 subfamily XXVIIB polypeptide 1
  • cytochrome P450, family 27, subfamily B, polypeptide 1
  • cytochrome P450, subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase)
  • Cytochrome P450C1 alpha
  • Cytochrome P450VD1-alpha
  • EC 1.14.13.13
  • P450c1
  • PDDR
  • polypeptide 1
  • VD3 1A hydroxylase
  • VDD1
  • VDDR
  • VDDRI
  • VDR

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CYP27B1