CYP27B1 Antibody

Product Discontinued

CYP27B1 Antibody Summary

• Immunogen: This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1.
• Localization: Cellular Location Mitochondrion membrane. Tissue Location Kidney.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP27B1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Western Blot 1:1000
• Theoretical MW: 56.504 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 6.9 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CYP27B1 Antibody

• 1alpha(OH)ase
• 25 hydroxyvitamin D3-1-alpha hydroxylase
• 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
• 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
• 25-OHD-1 alpha-hydroxylase
• Calcidiol 1-monooxygenase
• CP2B
• CYP1
• CYP1ALPHA
• CYP27B
• CYP27B1
• Cytochrome p450 27B1
• Cytochrome P450 subfamily XXVIIB polypeptide 1
• cytochrome P450, family 27, subfamily B, polypeptide 1
• cytochrome P450, subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase)
• Cytochrome P450C1 alpha
• Cytochrome P450VD1-alpha
• EC 1.14.13.13
• EC 1.14.15.18
• P450c1
• PDDR
• polypeptide 1
• VD3 1A hydroxylase
• VDD1
• VDDR
• VDDRI
• VDR

Background

CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYP27B1 Antibody (NBP2-29942)(1)

Publication using NBP2-29942

• White AD, PeNa KA, Clark LJ Et al. Spatial bias in cAMP generation determines biological responses to PTH type 1 receptor activation Science signaling 2021-10-05 [PMID: 34609896]

Bioinformatics

• Gene Symbol: CYP27B1
• Uniprot:
  • O15528(Human)