CYP27B1 Antibody

Images

 
Western Blot: CYP27B1 Antibody [NBP2-29942] - Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: T47D whole cell lysate Lane 3: Rat kidney lysate Lysates/proteins at 20 ug per lane. Secondary ...read more
Immunohistochemistry-Paraffin: CYP27B1 Antibody [NBP2-29942] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and ...read more
Flow Cytometry: CYP27B1 Antibody [NBP2-29942] - Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were ...read more
Western Blot: CYP27B1 Antibody [NBP2-29942] - Western blot analysis of (C-term) (NBP2-29942) in mouse kidney tissue lysates (35ug/lane). CYP27B1 (arrow) was detected using the purified Pab.
Western Blot: CYP27B1 Antibody [NBP2-29942] - Analysis of lysate from T47D cell line, using CYP27B1 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary ...read more
Western Blot: CYP27B1 Antibody [NBP2-29942] - Antibody (C-term) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
6.9 mg/ml

Order Details

CYP27B1 Antibody Summary

Immunogen
This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1.
Localization
Cellular Location Mitochondrion membrane. Tissue Location Kidney.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP27B1
Purity
Ammonium sulfate precipitation
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
Theoretical MW
56.504 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
6.9 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for CYP27B1 Antibody

  • 1alpha(OH)ase
  • 25 hydroxyvitamin D3-1-alpha hydroxylase
  • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
  • 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
  • 25-OHD-1 alpha-hydroxylase
  • Calcidiol 1-monooxygenase
  • CP2B
  • CYP1
  • CYP1alpha
  • CYP27B
  • Cytochrome p450 27B1
  • Cytochrome P450 subfamily XXVIIB polypeptide 1
  • cytochrome P450, family 27, subfamily B, polypeptide 1
  • cytochrome P450, subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase)
  • Cytochrome P450C1 alpha
  • Cytochrome P450VD1-alpha
  • EC 1.14.13.13
  • P450c1
  • PDDR
  • polypeptide 1
  • VD3 1A hydroxylase
  • VDD1
  • VDDR
  • VDDRI
  • VDR

Background

CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CYP27B1
Uniprot