Cyclin B2 Antibody - BSA Free

Western Blot: Cyclin B2 Antibody [NBP1-33031] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031] - Cyclin B2 Antibody detects Cyclin B2 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Cyclin B2 Antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031] - Paraffin-embedded H1299 xenograft; antibody dilution 1:500.

Cyclin B2 antibody [N1C3-2] detects CCNB2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: CCNB2 protein stained by Cyclin B2 antibody [N1C3-2] (NBP1-33031) diluted at 1:500.Blue: Hoechst 33343 staining.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cyclin B2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cyclin B2 Antibody - BSA Free (NBP1-33031) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cyclin B2. The exact sequence is proprietary.
• Specificity: human CCNB2 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCNB2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cyclin B2 Antibody - BSA Free

• CCNB2
• Cyclin B2
• G2/mitotic-specific cyclin-B2
• HsT17299

Background

Cyclin B2 belongs to a the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin B2 has been shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CCNB2