CXCR7/RDC-1 Antibody

Images

 
Simple Western: CXCR7/RDC-1 Antibody [NBP2-24779] - Simple Western lane view shows a specific band for CXCR7 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration of CXCR7/RDC-1 antibody. The staining was ...read more
Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration of CXCR7/RDC-1 antibody. The staining was ...read more
Western Blot: CXCR7/RDC-1 Antibody [NBP2-24779] - Analysis of human CXCR7/RDC1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Eq, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CXCR7/RDC-1 Antibody Summary

Immunogen
A synthetic peptide corresponding amino acids 106-129 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CXCR7
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 4-6 ug/ml
  • Simple Western 1:100
  • Flow Cytometry 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin 5-10 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Publications
Read Publications using
NBP2-24779 in the following applications:

Reactivity Notes

This antibody is reactive to Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for CXCR7/RDC-1 Antibody

  • ACKR3
  • chemokine (C-X-C motif) receptor 7
  • Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
  • CMKOR1
  • CXCR7
  • CXC-R7
  • CXCR-7
  • G protein-coupled receptor
  • GPR159
  • GPR159G-protein coupled receptor 159
  • RDC-1
  • RDC1G-protein coupled receptor RDC1 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR7/RDC-1 Antibody (NBP2-24779)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Flow-CS, WB.


Filter By Application
Flow-CS
(1)
WB
(2)
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Filter By Species
Human
(2)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 4 of 4.
Publications using NBP2-24779 Applications Species
Hattermann K, Held-Feindt J, Lucius R et al. The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects. Cancer Res. 2010 Apr 15 [PMID: 20388803] (WB, Human)

Details:
WB (Fig 2): human glioma cell lines UC118, A739, A761, A764, A767, and A777. CXCR7 was detected at 50 kDa.
WB Human
Odemis V, Boosmann K, Heinen A et al. CXCR7 is an active component of SDF-1 signalling in astrocytes and Schwann cells. J Cell Sci. 2010 Apr 1 [PMID: 20197403] (WB, Rat)

Details:
WB: Fig 1C (primary rat astrocytes transfected with CXCR7 siRNA or left untreated. Fig 1D (primary rat Schwann cells); Fig 1G (primary mouse glial and rat Schwann cells). Flow (cell surface): Figs 1E & F (primary rat astrocytes), Figs 2A & B (primary rat
WB Rat
Zhang Z, Zhong W, Hall MJ et al. CXCR4 but not CXCR7 is mainly implicated in ocular leukocyte trafficking during ovalbumin-induced acute uveitis. Exp Eye Res. 2009 Oct [PMID: 19524567] (Mouse)

Details:
FA (neutralization): In vivo FA assay: D011.10 mice [(intraperitoneal injection of 15 ug or intravitreal injection of 1.5 ug of CXCR7 pAb (IMG-6099A)], Fig 8. The mitigation of OVA-induced uveitis was assessed. The antibody did not significantly alter OVA
Mouse
Choy JC, Yi T, Rao DA et al. CXCL12 induction of inducible nitric oxide synthase in human CD8 T cells. J Heart Lung Transplant. 2008 Dec [PMID: 19059114] (Flow-CS, Human)

Details:
Flow (cell surface): freshly isolated human CD4 and CD8 cells (Fig 1) and HeLa cells (data described, but not shown). CXCR7 was detected in HeLa but not in CD4 or CD8 cells.
Flow-CS Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CXCR7
Entrez
Uniprot