CXCR7/RDC-1 Antibody

Simple Western: CXCR7/RDC-1 Antibody [NBP2-24779] - Lane view shows a specific band for CXCR7 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - Analysis of a FFPE tissue section of human tonsil using 5ug/ml concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.

Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - Analysis of a FFPE tissue section of human tonsil using 5ug/ml concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.

Western Blot: CXCR7/RDC-1 Antibody [NBP2-24779] - Total protein from human spleen, tonsil, lymph node and mouse spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CXCR7 in 1% BSA in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Sh, Bv, Eq, Pm
• Applications: WB, Simple Western, Flow, Flow-CS, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CXCR7/RDC-1 Antibody Summary

• Immunogen: A synthetic peptide corresponding amino acids 106-129 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1.
• Predicted Species: Bovine (100%), Equine (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACKR3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 4-6 ug/ml
  • Simple Western 1:100
  • Flow Cytometry 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Flow (Cell Surface)
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. Use in Flow-cell surface reported in scientific literature (PMID: 19059114).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CXCR7/RDC-1 Antibody

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR7/RDC-1 Antibody (NBP2-24779)(5)

Publications using NBP2-24779

• Quinn K, Prosser S, Kane K et al. Inhibition of chemokine (C-X-C motif) receptor four (CXCR4) at the fetal-maternal interface during early gestation in sheep: alterations in expression of chemokines, angiogenic factors and their receptors. J Anim Sci 2017 Mar 28 [PMID: 28380526] (WB, Sheep)
• Hattermann K, Held-Feindt J, Lucius R et al. The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects. Cancer Res. 2010 Apr 15 [PMID: 20388803] (WB, Human)
  
  Details:
  WB (Fig 2): human glioma cell lines UC118, A739, A761, A764, A767, and A777. CXCR7 was detected at 50 kDa.
• Odemis V, Boosmann K, Heinen A et al. CXCR7 is an active component of SDF-1 signalling in astrocytes and Schwann cells. J Cell Sci. 2010 Apr 1 [PMID: 20197403] (WB, Rat)
  
  Details:
  WB: Fig 1C (primary rat astrocytes transfected with CXCR7 siRNA or left untreated. Fig 1D (primary rat Schwann cells); Fig 1G (primary mouse glial and rat Schwann cells). Flow (cell surface): Figs 1E & F (primary rat astrocytes), Figs 2A & B (primary rat
• Zhang Z, Zhong W, Hall MJ et al. CXCR4 but not CXCR7 is mainly implicated in ocular leukocyte trafficking during ovalbumin-induced acute uveitis. Exp Eye Res. 2009 Oct [PMID: 19524567] (Mouse)
  
  Details:
  FA (neutralization): In vivo FA assay: D011.10 mice [(intraperitoneal injection of 15 ug or intravitreal injection of 1.5 ug of CXCR7 pAb (IMG-6099A)], Fig 8. The mitigation of OVA-induced uveitis was assessed. The antibody did not significantly alter OVA
• Choy JC, Yi T, Rao DA et al. CXCL12 induction of inducible nitric oxide synthase in human CD8 T cells. J Heart Lung Transplant. 2008 Dec [PMID: 19059114] (Flow-CS, Human)
  
  Details:
  Flow (cell surface): freshly isolated human CD4 and CD8 cells (Fig 1) and HeLa cells (data described, but not shown). CXCR7 was detected in HeLa but not in CD4 or CD8 cells.

Bioinformatics

• Gene Symbol: ACKR3
• Uniprot:
  • P25106()