RAMP2 Antibody (OTI4E5)

Western Blot: RAMP2 Antibody (4E5) [NBP2-01853] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAMP2.

Immunohistochemistry-Paraffin: RAMP2 Antibody (4E5) [NBP2-01853] - FFPE gingival tissue from Macaca mulatta was stained with RAMP2 Antibody (4E5) as described in

Flow Cytometry: RAMP2 Antibody (4E5) [NBP2-01853] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAMP2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: RAMP2 Antibody (4E5) [NBP2-01853] - Staining of paraffin-embedded Human Kidney tissue using anti-RAMP2 mouse monoclonal antibody.

• Reactivity: Hu, Pm
• Applications: WB, Flow, IHC, IHC-P
• Clone: OTI4E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RAMP2 Antibody (OTI4E5) Summary

• Immunogen: Full length human recombinat protein of human RAMP2(NP_005845) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAMP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000
• Theoretical MW: 15.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAMP2 Antibody (OTI4E5)

• calcitonin receptor-like receptor activity modifying protein 2
• Calcitonin-receptor-like receptor activity-modifying protein 2
• CRLR activity-modifying protein 2
• RAMP2
• receptor (calcitonin) activity modifying protein 2
• receptor (G protein-coupled) activity modifying protein 2
• receptor activity modifying protein 2
• receptor activity-modifying protein 2
• receptor-activity-modifying protein 2

Background

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for RAMP2 Antibody (NBP2-01853) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Macaca mulatta.

reviewed by: Ahmad Al-Attar IHC-P Macaca mulatta 02/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Gingival tissue
• Species: Macaca mulatta
• Lot: A002

Comments

• Comments: HIER: Diva Decloaker 5 minutes + 45 minutes cool down
  Antibody dilution: 1:150
  Antibody incubation: Overnight @ 4 deg C
  DAB: 5 min
  
  Please use this review for an Innovators Reward Program.
  Also, please use code MBCS17 (to get an additional 100 points per review). Thank you!

Bioinformatics

• Gene Symbol: RAMP2
• Entrez:
  • 10266(Human)