MAB4227) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm. Protocol available in
COMET™ Panel Builder." class="big_lightbox">

MAB4227) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm. Protocol available in
COMET™ Panel Builder." alt="CXCR7 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human CXCR7 Monoclonal Antibody (Catalog #
MAB4227) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the cytoplasm. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B)." class="big_lightbox">

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B)." class="big_thumb" />