CXCR6 Antibody (56811) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) treated with 150 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days were stained with Mouse Anti-Human CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB699P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (IC0041P). View our protocol for Staining Membrane-associated Proteins.

HEK293 cells transfected with Human CXCR6 and eGFP (A) vs irrelevant and eGFP (B) were stained with Mouse Anti-Human CXCR6 PE‑conjugated Monoclonal Antibody (Catalog # FAB699P) at 37°C. View our protocol for Staining Membrane-associated Proteins.

CXCR6 and CXCL16 expression in LuCa cell lines(A) mRNA levels by semiquantitative RT-PCR. The copies of CXCR6 and CXCL16 transcripts are expressed relative to copies of 18S rRNA. Values are mean ± SEM from 3 independent experiments. *p ≤ 0.05, ***p ≤ 0.001 as compared to SCC (NCI-H520). (B) Flow cytometry analysis of CXCR6 and trans-membrane CXCL16 in (i) - SCC (NCI-H520) cells and (ii) - AC (NCI-H2126) cells. Grey dots represent isotype controls for PE and APC antibody and black dots represent CXCR6-PE and CXCL16-APC in SCC (NCI-H520) and AC (NCI-H2126). CXCR6 and CXCL16 both are expressed on ~82.9% (Q2) SCC cells; 5.41% (Q1) express only CXCR6 and only CXCL16 is expressed by 9.08% (Q3) of SCC cells. In AC cells, 61.7% of the population express both receptor and ligand (Q2) whereas, 11.1 (Q1) and 16.2% (Q3) cells express only CXCR6 and CXCL16, respectively. c) Levels of soluble CXCL16 in LuCa supernatant. Values are mean ± SEM from 3 independent experiments. ***p ≤ 0.0001 compared to NCI-H520. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.3194), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 56811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CXCR6 Antibody (56811) [Phycoerythrin] Summary

• Additional Information: Clone 56811 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR6
  Met1-Leu342
  Accession # O00574
• Specificity: Stains human CXCR6 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR6
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR6 Antibody (56811) [Phycoerythrin]

• Bonzo
• BONZOG-protein coupled receptor bonzo
• CD186 antigen
• CD186
• CDw186
• chemokine (C-X-C motif) receptor 6
• C-X-C chemokine receptor type 6
• CXCR6
• CXC-R6
• CXCR-6
• G protein-coupled receptor
• STRL33
• STRL33G-protein coupled receptor STRL33
• TYMSTRCD186

Background

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR6 Antibody (FAB699P)(19)

Publications using FAB699P

• Maria Rita Fumagalli, Maria Chiara Lionetti, Stefano Zapperi, Caterina A. M. La Porta Cross-Talk Between circRNAs and mRNAs Modulates MiRNA-mediated Circuits and Affects Melanoma Plasticity Cancer Microenvironment 12/1/2019 [PMID: 31734859]
• Lepore F, D'Alessandro G, Antonangeli F et al. CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma Front Immunol 11/27/2018 [PMID: 30542347]
• Stuart Hunter, Carrie R. Willcox, Martin S. Davey, Sofya A. Kasatskaya, Hannah C. Jeffery, Dmitriy M. Chudakov et al. Human liver infiltrating gamma δ T cells are composed of clonally expanded circulating and tissue-resident populations Journal of Hepatology 9/1/2018 [PMID: 29758330]
• Stuart Hunter, Carrie R. Willcox, Martin S. Davey, Sofya A. Kasatskaya, Hannah C. Jeffery, Dmitriy M. Chudakov et al. Human liver infiltrating gamma δ T cells are composed of clonally expanded circulating and tissue-resident populations Journal of Hepatology 2018-09-01 [PMID: 29758330] (Flow Cytometry, Human)
• A Koenen, A Babendreye, J Schumacher, T Pasqualon, N Schwarz, A Seifert, X Deupi, A Ludwig, D Dreymuelle The DRF motif of CXCR6 as chemokine receptor adaptation to adhesion PLoS ONE, 2017-03-07;12(3):e0173486. 2017-03-07 [PMID: 28267793] (Flow Cytometry, Human)
• Lee Faulkner Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity Toxicol. Sci, 2016-09-16;154(2):416-429. 2016-09-16 [PMID: 27637899] (Flow Cytometry, Human)
• Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways. J Autoimmun, 2015-08-30;66(0):40-50. 2015-08-30 [PMID: 26330348] (Flow Cytometry, Human)
• Hina Mir, Rajesh Singh, Goetz H. Kloecker, James W. Lillard, Shailesh Singh CXCR6 expression in non-small cell lung carcinoma supports metastatic process via modulating metalloproteinases Oncotarget 2015-04-30 [PMID: 25888629] (Flow Cytometry, Human)
• Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines. PLoS ONE, 2015-03-16;10(3):e0120069. 2015-03-16 [PMID: 25774696] (Flow Cytometry, Human)
• Lee , Woo-Yong, Sanz , Maria-Je, Wong , Connie H, Hardy , Pierre-O, Salman-Dilgimen , Aydan, Moriarty , Tara J, Chaconas , George, Marques , Adriana, Krawetz , Roman, Mody , Christop, Kubes , Paul Invariant natural killer T cells act as an extravascular cytotoxic barrier for joint-invading Lyme Borrelia. Proc Natl Acad Sci U S A, 2014-09-09;111(38):13936-41. 2014-09-09 [PMID: 25205813] (IHC, Human)
• Hu , Weidong, Liu , Yue, Zhou , Wenhui, Si , Lianlian, Ren , Liang CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro. PLoS ONE, 2014-06-04;9(6):e99056. 2014-06-04 [PMID: 24897301] (Flow Cytometry, Human)
• Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis. Clin. Exp. Immunol., 2010-11-19;163(2):215-24. 2010-11-19 [PMID: 21087446] (Flow Cytometry, Human)
• Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood, 2010-11-17;117(4):1250-9. 2010-11-17 [PMID: 21084709] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Campillo-Gimenez L, Cumont MC, Fay M, Kared H, Monceaux V, Diop O, Muller-Trutwin M, Hurtrel B, Levy Y, Zaunders J, Dy M, Leite-de-Moraes MC, Elbim C, Estaquier J AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection. J. Immunol., 2009-12-16;184(2):984-92. 2009-12-16 [PMID: 20018630] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections. Clin. Exp. Immunol., 2008-09-05;154(2):216-23. 2008-09-05 [PMID: 18778363] (Flow Cytometry, Human)
• Martini G, Cabrelle A, Calabrese F, Carraro S, Scquizzato E, Teramo A, Facco M, Zulian F, Agostini C CXCR6-CXCL16 interaction in the pathogenesis of Juvenile Idiopathic Arthritis. Clin. Immunol., 2008-08-28;129(2):268-76. 2008-08-28 [PMID: 18760678] (Flow Cytometry, ICC, Human)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007-09-15;179(6):4272-82. 2007-09-15 [PMID: 17785868] (Flow Cytometry, Human)
• Freeman CM, Curtis JL, Chensue SW CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity. Am. J. Pathol., 2007-07-19;171(3):767-76. 2007-07-19 [PMID: 17640964] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CXCR6
• Uniprot:
  • O00574()