CXCR6 Antibody (56811) [Allophycocyanin]

HEK293 human embryonic kidney cell line transfected with human CXCR6 was stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 9 days were stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 56811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CXCR6 Antibody (56811) [Allophycocyanin] Summary

• Additional Information: Clone 56811 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR6
  Met1-Leu342
  Accession # O00574
• Specificity: Stains human CXCR6 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR6
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR6 Antibody (56811) [Allophycocyanin]

• Bonzo
• BONZOG-protein coupled receptor bonzo
• CD186 antigen
• CD186
• CDw186
• chemokine (C-X-C motif) receptor 6
• C-X-C chemokine receptor type 6
• CXCR6
• CXC-R6
• CXCR-6
• G protein-coupled receptor
• STRL33
• STRL33G-protein coupled receptor STRL33
• TYMSTRCD186

Background

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR6 Antibody (FAB699A)(6)

Publications using FAB699A

• D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response Proc Natl Acad Sci U S A, 2020-10-30;0(0):. 2020-10-30 [PMID: 33127761] (Flow Cytometry, Human)
• J Cantero-Pé, J Grau-Expós, C Serra-Pein, DA Rosero, L Luque-Ball, A Astorga-Ga, J Castellví, T Sanhueza, G Tapia, B Lloveras, MA Fernández, JG Prado, JM Solé-Seden, A Tarrats, C Lecumberri, L Mañalich-B, C Centeno-Me, V Falcó, MJ Buzon, M Genescà Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa Nat Commun, 2019-10-18;10(1):4739. 2019-10-18 [PMID: 31628331] (Flow Cytometry, Human)
• E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis Arthritis Res. Ther., 2018-06-07;20(1):114. 2018-06-07 [PMID: 29880013] (Flow Cytometry, Human)
• Morandi , Fabio, Di Carlo , Emma, Ferrone , Soldano, Petretto , Andrea, Pistoia , Vito, Airoldi , Irma IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol, 2014-02-19;192(6):2634-42. 2014-02-19 [PMID: 24554774] (Flow Cytometry, Human)
• Ahern D, Lloyd CM, Robinson DS Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors. Allergy, 2009-02-07;64(8):1121-9. 2009-02-07 [PMID: 19208087] (Flow Cytometry, Human)
• Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections. Clin. Exp. Immunol., 2008-09-05;154(2):216-23. 2008-09-05 [PMID: 18778363] (Flow Cytometry, IHC-P, Human)

Reviews for CXCR6 Antibody (FAB699A) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/27/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 21203549
• Species: Human

reviewed by: Verified Customer Flow Human 01/27/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 21203549
• Species: Human

Bioinformatics

• Gene Symbol: CXCR6
• Uniprot:
  • O00574()