CX3CR1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of M1 mouse myeloid leukemia cell line and NK‑92 human natural killer lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal ...read more
M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

CX3CR1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9
Specificity
Detects human and mouse CX3CR1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CX3CR1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF5825 in the following applications:

Publications
Read Publications using
AF5825 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CR1 Antibody [Unconjugated]

  • Beta chemokine receptor-like 1
  • CCRL1
  • chemokine (C-C) receptor-like 1
  • chemokine (C-X3-C motif) receptor 1
  • chemokine (C-X3-C) receptor 1
  • CMKbRL1
  • CMK-BRL1
  • CMK-BRL-1
  • CMKDR1
  • CX3C chemokine receptor 1
  • CX3CR1
  • Fractalkine receptor
  • G protein-coupled receptor 13
  • GPR13G-protein coupled receptor 13
  • GPRV28
  • V28
  • V28C-X3-C CKR-1

Background

CX3CR1 (CX3C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa (predicted) member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CX3CR1 Antibody (AF5825)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, In Vivo, Western Blot.


Filter By Application
Flow Cytometry
(4)
ICC
(1)
IHC
(1)
IHC-Fr
(2)
In Vivo
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF5825 Applications Species
Long, J;Liu, H;Qiu, Z;Xiao, Z;Lu, Z; Glabridin Therapy Reduces Chronic Allodynia, Spinal Microgliosis, and Dendritic Spine Generation by Inhibiting Fractalkine-CX3CR1 Signaling in a Mouse Model of Tibial Fractures Brain sciences 2023-04-29 [PMID: 37239211] (In Vivo, Mouse) In Vivo Mouse
T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment Oncotarget, 2018-01-29;9(13):11209-11226. 2018-01-29 [PMID: 29541408] (Flow Cytometry, Mouse) Flow Cytometry Mouse
KH Han, BM Arlian, MS Macauley, JC Paulson, RA Lerner Migration-based selections of antibodies that convert bone marrow into trafficking microglia-like cells that reduce brain amyloid ? Proc. Natl. Acad. Sci. U.S.A., 2018-01-02;0(0):. 2018-01-02 [PMID: 29295920] (IHC, Mouse) IHC Mouse
T Yano, M Ohira, R Nakano, Y Tanaka, H Ohdan Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice PLoS ONE, 2017-10-31;12(10):e0186997. 2017-10-31 [PMID: 29088306] (Flow Cytometry, Mouse) Flow Cytometry Mouse
K Mussar, S Pardike, TM Hohl, G Hardiman, V Cirulli, L Crisa A CCR2+ myeloid cell niche required for pancreatic ? cell growth JCI Insight, 2017-08-03;2(15):. 2017-08-03 [PMID: 28768911] (Flow Cytometry, Mouse) Flow Cytometry Mouse
R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake MicroRNA-101a regulates microglial morphology and inflammation J Neuroinflammation, 2017-05-30;14(1):109. 2017-05-30 [PMID: 28558818] (ICC, Mouse) ICC Mouse
Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology Alzheimers Res Ther, 2016-12-15;8(1):54. 2016-12-15 [PMID: 27974048] (IHC-Fr, Mouse) IHC-Fr Mouse
MKEY, a Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, Protects Against Stroke in Mice J Am Heart Assoc, 2016-09-15;5(9):. 2016-09-15 [PMID: 27633389] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Kowalewska P, Patrick A, Fox-Robichaud A Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation. PLoS ONE, 2014-09-03;9(9):e104537. 2014-09-03 [PMID: 25184228] (IHC-Fr, Mouse) IHC-Fr Mouse
Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP Fractalkine is a novel human adipochemokine associated with type 2 diabetes. Diabetes, 2011-05-01;60(5):1512-8. 2011-05-01 [PMID: 21525510] (Western Blot, Human) Western Blot Human

Review for CX3CR1 Antibody (AF5825) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF5825:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21888931
SpeciesHuman

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FAQs for CX3CR1 Antibody (AF5825) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on CX3CR1.

TMEM 119 is a specific marker of microglia cells
By Jennifer Sokolowski, MD, PhD.Microglia are a major immune-cell component in the brain. They ingest and degrade dead cells, debris, and foreign material and interact with other immune cells to orchestrate centra...  Read full blog post.

New Study Links Tau Mutations to Microglial Immune Response
Tau proteins are abundant in the axons of neurons in the central nervous system (CNS), and play a key role in microtubule formation and stabilization. Antibody studies have identified six tau isoforms, all produced by alternative mRNA splicing of the ...  Read full blog post.

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Verified Customer
01/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CX3CR1
Uniprot