CX3CR1 Antibody [Unconjugated]

Western blot shows lysates of M1 mouse myeloid leukemia cell line and NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CX3CR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu, Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CX3CR1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CX3CR1
  Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
  Accession # Q9Z0D9
• Specificity: Detects human and mouse CX3CR1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CX3CR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CR1 Antibody [Unconjugated]

• Beta chemokine receptor-like 1
• CCRL1
• chemokine (C-C) receptor-like 1
• chemokine (C-X3-C motif) receptor 1
• chemokine (C-X3-C) receptor 1
• CMKbRL1
• CMK-BRL1
• CMK-BRL-1
• CMKDR1
• CX3C chemokine receptor 1
• CX3CR1
• Fractalkine receptor
• G protein-coupled receptor 13
• GPR13G-protein coupled receptor 13
• GPRV28
• V28
• V28C-X3-C CKR-1

Background

CX3CR1 (CX₃C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa (predicted) member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1^lo monocytes plus smooth muscle and mast cells. CX₃CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX₃CR1 shares 96% and 77% aa identity with rat and human CX₃CR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CR1 Antibody (AF5825)(17)

Publications using AF5825

• Evans, LMP;Gawron, J;Sim, FJ;Feltri, ML;Marziali, LN; Human iPSC-derived myelinating organoids and globoid cells to study Krabbe disease PloS one 2024-12-05 [PMID: 39636943] (Immunocytochemistry, Human)
• Evans, LMP;Gawron, J;Sim, FJ;Laura Feltri, M;Marziali, LN; Human iPSC-derived myelinating organoids and globoid cells to study Krabbe Disease bioRxiv : the preprint server for biology 2024-07-23 [PMID: 39091729] (Immunohistochemistry, Immunocytochemistry, Human)
• Long, J;Liu, H;Qiu, Z;Xiao, Z;Lu, Z; Glabridin Therapy Reduces Chronic Allodynia, Spinal Microgliosis, and Dendritic Spine Generation by Inhibiting Fractalkine-CX3CR1 Signaling in a Mouse Model of Tibial Fractures Brain sciences 2023-04-29 [PMID: 37239211] (In Vivo, Mouse)
• Chia‐Yu Wu, Pei‐Wen Peng, Ting‐Yi Renn, Chia‐Jung Lee, Tsung‐Ming Chang, Augusta I‐Chin Wei et al. CX3CL1 induces cell migration and invasion through ICAM‐1 expression in oral squamous cell carcinoma cells Journal of Cellular and Molecular Medicine 2023-06-01 [PMID: 37082943]
• Kurt Brassington, Peter Kanellakis, Anh Cao, Ban-Hock Toh, Karlheinz Peter, Alex Bobik et al. Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts Frontiers in Immunology 2022-11-22 [PMID: 36483558]
• Jingjing Zhang, Yufei Liu, Xinyao Liu, Song Li, Cheng Cheng, Sheng Chen et al. Dynamic changes of CX3CL1/CX3CR1 axis during microglial activation and motor neuron loss in the spinal cord of ALS mouse model Translational Neurodegeneration 2018-12-21 [PMID: 30607245]
• T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment Oncotarget, 2018-01-29;9(13):11209-11226. 2018-01-29 [PMID: 29541408] (Flow Cytometry, Mouse)
• KH Han, BM Arlian, MS Macauley, JC Paulson, RA Lerner Migration-based selections of antibodies that convert bone marrow into trafficking microglia-like cells that reduce brain amyloid ? Proc. Natl. Acad. Sci. U.S.A., 2018-01-02;0(0):. 2018-01-02 [PMID: 29295920] (IHC, Mouse)
• T Yano, M Ohira, R Nakano, Y Tanaka, H Ohdan Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice PLoS ONE, 2017-10-31;12(10):e0186997. 2017-10-31 [PMID: 29088306] (Flow Cytometry, Mouse)
• K Mussar, S Pardike, TM Hohl, G Hardiman, V Cirulli, L Crisa A CCR2+ myeloid cell niche required for pancreatic ? cell growth JCI Insight, 2017-08-03;2(15):. 2017-08-03 [PMID: 28768911] (Flow Cytometry, Mouse)
• R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake MicroRNA-101a regulates microglial morphology and inflammation J Neuroinflammation, 2017-05-30;14(1):109. 2017-05-30 [PMID: 28558818] (ICC, Mouse)
• Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology Alzheimers Res Ther, 2016-12-15;8(1):54. 2016-12-15 [PMID: 27974048] (IHC-Fr, Mouse)
• MKEY, a Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, Protects Against Stroke in Mice J Am Heart Assoc, 2016-09-15;5(9):. 2016-09-15 [PMID: 27633389] (Flow Cytometry, Mouse)
• Kowalewska P, Patrick A, Fox-Robichaud A Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation. PLoS ONE, 2014-09-03;9(9):e104537. 2014-09-03 [PMID: 25184228] (IHC-Fr, Mouse)
• Wen-Hui Tsai, Chung-Hung Shih, Shan-Yu Feng, I-Ting Li, Shao-Chi Chang, Yu-Chieh Lin et al. CX3CL1(+) Microparticles Mediate the Chemoattraction of Alveolar Macrophages toward Apoptotic Acute Promyelocytic Leukemic Cells Cellular Physiology and Biochemistry 2014-01-20 [PMID: 24603149]
• Soehnlein Oliver, Drechsler Maik, Doring Yvonne et al. Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes. EMBO Mol Med. 3/1/2013 [PMID: 23417922]
• Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP Fractalkine is a novel human adipochemokine associated with type 2 diabetes. Diabetes, 2011-05-01;60(5):1512-8. 2011-05-01 [PMID: 21525510] (Western Blot, Human)

Review for CX3CR1 Antibody (AF5825) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21888931
• Species: Human

Bioinformatics

• Gene Symbol: CX3CR1
• Uniprot:
  • Q9Z0D9()