CXCR5 Antibody (614641) [Allophycocyanin]

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Mouse T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

The percentages of CD4+CXCR5+ICOS5+ T cells in peripheral blood of patients with T1D.Peripheral blood mononuclear cells (PMBCs) from T1D patients (n = 54) and healthy controls (n = 31) were stained with labelled antibodies as described in Methods. A. Representative dot plots of CD4+CXCR5+ICOS5+ T cells in different groups of samples. Values in the upper right quadrant correspond to the percentages of CD4+CXCR5+ICOS5+ T cells. At least about 50,000 events were analyzed for each sample. B. CD4+CXCR5+ICOS5+ T cells were compared between T1D patients and healthy controls. Each data point represents an individual subject. The bars indicate the mean values. Student's unpaired t test was performed. ***, P<0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24278195), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: Flow
• Clone: 614641
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

CXCR5 Antibody (614641) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant mouse CXCR5
  Met1-Pro57
  Accession # Q04683
• Specificity: Detects recombinant mouse (rm) CXCR5 in direct ELISAs. In this format, no cross-reactivity with rmCXCR7 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR5 Antibody (614641) [Allophycocyanin]

• BLR1
• BLR1MDR-15
• Burkitt lymphoma receptor 1
• Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
• Burkitt lymphoma receptor 1, GTP-binding protein
• CD185 antigen
• CD185
• chemokine (C-X-C motif) receptor 5
• C-X-C chemokine receptor type 5
• CXCR5
• CXC-R5
• MDR15CXCR-5
• MGC117347
• Monocyte-derived receptor 15

Background

CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4⁺ CD3^- IL-7R alpha ⁺ precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR5 Antibody (FAB6198A)(2)

Publications using FAB6198A

• Madhav D. Sharma, Rafal Pacholczyk, Huidong Shi, Zuzana J. Berrong, Yousef Zakharia, Austin Greco et al. Inhibition of a BTK-IDO-mTOR axis promotes differentiation of monocyte-lineage inflammatory dendritic cells and enhances anti-tumor T cell immunity Immunity 2021-10-01 [PMID: 34614413]
• Xu , Xinyu, Shi , Yun, Cai , Yun, Zhang , Qingqing, Yang , Fan, Chen , Heng, Gu , Yong, Zhang , Mei, Yu , Liping, Yang , Tao Inhibition of increased circulating Tfh cell by anti-CD20 monoclonal antibody in patients with type 1 diabetes. PLoS ONE, 2013-11-20;8(11):e79858. 2013-11-20 [PMID: 24278195] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CXCR5
• Uniprot:
  • Q04683()