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CXCR5 Antibody (614641) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
614641
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
FAB6198V-100UG
Alexa Fluor 488
FAB6198G-100UG
Alexa Fluor 594
FAB6198T-100UG
Alexa Fluor 647
FAB6198R-100UG
Alexa Fluor 700
FAB6198N-100UG
Alexa Fluor 750
FAB6198S-100UG
Allophycocyanin
FAB6198A-100UG
Unconjugated
MAB6198
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6198
Carrier Free
MAB6198
Carrier Free
MAB6198-SP
Carrier Free
FAB6198V-100UG
Carrier Free
FAB6198T-100UG
Carrier Free
FAB6198S-100UG
Carrier Free
FAB6198R-100UG
Carrier Free
FAB6198N-100UG
Carrier Free
FAB6198G-100UG
Carrier Free
FAB6198A-100UG
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CXCR5 Antibody (614641) [Alexa Fluor® 350] Summary

Immunogen
E. coli-derived recombinant mouse CXCR5
Met1-Pro57
Accession # Q04683
Specificity
Detects recombinant mouse CXCR5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CXCR7 is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CXCR5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR5 Antibody (614641) [Alexa Fluor® 350]

  • BLR1
  • BLR1MDR-15
  • Burkitt lymphoma receptor 1
  • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
  • Burkitt lymphoma receptor 1, GTP-binding protein
  • CD185 antigen
  • CD185
  • chemokine (C-X-C motif) receptor 5
  • C-X-C chemokine receptor type 5
  • CXCR5
  • CXC-R5
  • MDR15CXCR-5
  • MGC117347
  • Monocyte-derived receptor 15

Background

CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCL13/BLC/BCA‑1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA‑1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND50 is typically 20-50 µg/mL.
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CCR7 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
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IL-21
[Unconjugated]
594-ML/CF
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ICOS [Unconjugated]
169-CS
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CTLA-4
[Unconjugated]
7268-CT
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MAB160
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6507-IL/CF
IL-4 [Unconjugated]
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MAB182
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(53103)
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IL-22
[Unconjugated]
782-IL/CF
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CCRL2/LCCR
Antibody (498321)
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CCL21/6Ckine
[Unconjugated]
366-6C/CF
CCL21/6Ckine [Unconjugated]
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TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Fumarase Antibody
NBP1-31336
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Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-31336] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody diluted at 1:500.Blue: Hoechst 33342 staining.
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Complement Factor
H Antibody [Unconjuga ...
AF4779
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB6198U
Alexa Fluor 405 FAB6198V
Alexa Fluor 488 FAB6198G
Alexa Fluor 594 FAB6198T
Alexa Fluor 647 FAB6198R
Alexa Fluor 700 FAB6198N
Alexa Fluor 750 FAB6198S
Allophycocyanin FAB6198A
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CXCR5
Uniprot