CXCR5 Antibody (51505) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 51505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CXCR5 Antibody (51505) [Phycoerythrin] Summary

• Additional Information: Clone 51505 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR5
  Met1-Phe372
  Accession # P32302
• Specificity: Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR5 Antibody (51505) [Phycoerythrin]

• BLR1
• BLR1MDR-15
• Burkitt lymphoma receptor 1
• Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
• Burkitt lymphoma receptor 1, GTP-binding protein
• CD185 antigen
• CD185
• chemokine (C-X-C motif) receptor 5
• C-X-C chemokine receptor type 5
• CXCR5
• CXC-R5
• MDR15CXCR-5
• MGC117347
• Monocyte-derived receptor 15

Background

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR5 Antibody (FAB190P)(25)

Publications using FAB190P

• M Bunse, J Pfeilschif, J Bluhm, M Zschummel, JJ Joedicke, A Wirges, H Stark, V Kretschmer, M Chmielewsk, W Uckert, H Abken, J Westermann, A Rehm, UE Höpken CXCR5 CAR-T cells simultaneously target B cell non-Hodgkin's lymphoma and tumor-supportive follicular T helper cells Nature Communications, 2021-01-11;12(1):240. 2021-01-11 [PMID: 33431832] (ICC, IHC, Human, Xenograft)
• V Cunill, A Clemente, N Lanio, C Barceló, V Andreu, J Pons, JM Ferrer Follicular T Cells from smB(-) Common Variable Immunodeficiency Patients Are Skewed Toward a Th1 Phenotype Front Immunol, 2017-02-27;8(0):174. 2017-02-27 [PMID: 28289412] (Flow Cytometry, Human)
• A distinct plasmablast and na�ve B-cell phenotype in primary immune thrombocytopenia Haematologica, 2016-03-11;101(6):698-706. 3/11/2016 [PMID: 26969086]
• Paolo Carrega, Fabrizio Loiacono, Emma Di Carlo, Angelo Scaramuccia, Marco Mora, Romana Conte et al. NCR(+)ILC3 concentrate in human lung cancer and associate with intratumoral lymphoid structures Nature Communications 2015-09-23 [PMID: 26395069]
• Seok Jin Kim, Kyung Ju Ryu, Mineui Hong, Young Hyeh Ko, Won Seog Kim The serum CXCL13 level is associated with the Glasgow Prognostic Score in extranodal NK/T-cell lymphoma patients Journal of Hematology & Oncology 2015-12-01 [PMID: 25966773]
• Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines. PLoS ONE, 2015-03-16;10(3):e0120069. 2015-03-16 [PMID: 25774696] (Flow Cytometry, Human)
• Martinez-Martin N, Viejo-Borbolla A, Martin R, Blanco S, Benovic J, Thelen M, Alcami A Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization. Nat Commun, 2015-01-27;6(0):6163. 2015-01-27 [PMID: 25625471] (Flow Cytometry, Human)
• Morandi , Fabio, Di Carlo , Emma, Ferrone , Soldano, Petretto , Andrea, Pistoia , Vito, Airoldi , Irma IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol, 2014-02-19;192(6):2634-42. 2014-02-19 [PMID: 24554774] (Flow Cytometry, Human)
• Lehmann , Dorit, Spanholtz , Jan, Sturtzel , Caterina, Tordoir , Marleen, Schlechta , Bernhard, Groenewegen , Dirk, Hofer , Erhard IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential. PLoS ONE, 2014-01-31;9(1):e87131. 2014-01-31 [PMID: 24498025] (Flow Cytometry, Human)
• Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011-09-01;6(9):e23741. 2011-09-01 [PMID: 21912642] (Flow Cytometry, Human)
• Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation. PLoS ONE, 2011-08-03;6(8):e22848. 2011-08-03 [PMID: 21826211] (Flow Cytometry, Human)
• Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis. Clin. Exp. Immunol., 2010-11-19;163(2):215-24. 2010-11-19 [PMID: 21087446] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection. Immunology, 2009-08-01;127(4):549-57. 2009-08-01 [PMID: 19604305] (Flow Cytometry, Human)
• Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br. J. Cancer, 2008-09-16;99(6):930-8. 2008-09-16 [PMID: 18781150] (Flow Cytometry, Human)
• Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 2008-09-09;112(12):4401-10. 2008-09-09 [PMID: 18780835] (Flow Cytometry, Human)
• Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals. J. Exp. Med., 2008-07-14;205(8):1797-805. 2008-07-14 [PMID: 18625747] (Flow Cytometry, Human)
• Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation. J. Neuroimmunol., 2008-06-09;199(1):35-45. 2008-06-09 [PMID: 18538864] (Flow Cytometry, Human)
• Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1. Cancer Res., 2008-02-01;68(3):790-9. 2008-02-01 [PMID: 18245480] (Flow Cytometry, Human)
• Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells. Blood, 2008-01-02;111(5):2744-54. 2008-01-02 [PMID: 18172003] (Flow Cytometry, Human)
• Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia. Blood, 2007-07-25;110(9):3316-25. 2007-07-25 [PMID: 17652619] (Flow Cytometry, Human)
• Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J. Immunol., 2007-03-01;178(5):2872-82. 2007-03-01 [PMID: 17312131] (Flow Cytometry, Human)
• Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy. Int. J. Cancer, 2006-05-01;118(9):2147-57. 2006-05-01 [PMID: 16331601] (Flow Cytometry, Human)
• Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation. Blood, 2004-09-28;105(3):931-9. 2004-09-28 [PMID: 15454484] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-05-01;73(5):584-90. 2003-05-01 [PMID: 12714572] (Flow Cytometry, Human)

Reviews for CXCR5 Antibody (FAB190P) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 22367177
• Species: Human

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 22367177
• Species: Human

Bioinformatics

• Gene Symbol: CXCR5
• Uniprot:
  • P32302()