CXCR4 Antibody

Product Discontinued

CXCR4 Antibody Summary

• Immunogen: Synthetic peptide YSEEVGSGDYDSNKEPCFRDENVHFNR corresponding to amino acids 14-40 within the N terminal region of mouse CD184.
• Specificity: NBP1-21437 recognizes an epitope within the N-terminal (NT) region of mouse CD184, otherwise known as CXCR4 (C-X-C chemokine receptor type 4), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, ubiquitously expressed in blood and tissue cells, which acts as a specific receptor for the chemokine SDF-1 (Stromal cell-derived factor 1). Interaction of CD184 with SDF-1 mediates the chemotaxis of progenitor and mature blood cells, and is critical for B-lymphopoiesis and myelopoiesis. CD184 is a major co-receptor for infection by T-cell tropic strains of HIV1, and also a primary receptor for some HIV2 isolates. NBP1-21437 is reported as suitable for use in immunocytochemistry on mouse spleen leucocytes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:50000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:300
  • Western Blot 1:1000
• Application Notes: Feedback on frozen sections has been negative. Other histochemical applications have worked.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CXCR4 Antibody

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The a chemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV1 and HIV2 fusion and infection of human target cells. The amino terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CXCR4 Antibody (NBP1-21437). (Showing 1 - 1 of 1 FAQ).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Entrez:
  • 12767(Mouse)
• Uniprot:
  • P70658()